Pseudomonas aeruginosa PAO1, PA2887 (atuB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA2887
Name	atuB
Replicon	chromosome
Genomic location	3239711 - 3240589 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment
Comment	Essential for catabolism of acyclic terpenes (shown by knock-out).

Cross-References

RefSeq	NP_251577.1
GI	15598083
Affymetrix	PA2887_at
DNASU	PaCD00007263
Entrez	882609
GenBank	AAG06275.1
INSDC	AAG06275.1
NCBI Locus Tag	PA2887
protein_id(GenBank)	gb|AAG06275.1|AE004715_1|gnl|PseudoCAP|PA2887
TIGR	NTL03PA02887
UniParc	UPI00000C5862
UniProtKB Acc	Q9HZW0
UniProtKB ID	Q9HZW0_PSEAE
UniRef100	UniRef100_A0A072ZT86
UniRef50	UniRef50_D0CG44
UniRef90	UniRef90_W0YSQ8

Product

Feature Type	CDS
Coding Frame	1
Product Name	AtuB Product Name Confidence: Class 1
Synonyms	putative dehydrogenase involved in catabolism of citronellol
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	1.10
Kyte-Doolittle Hydrophobicity Value	-0.028
Molecular Weight (kDa)	30.8
Isoelectric Point (pI)	7.32

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6LLN	OXIDOREDUCTASE	12/23/19	citronellol catabolism dehydrogenase (AtuB) [Pseudomonas aeruginosa PAO1]	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.8	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 650 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002686 (393 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA2887 Search term: atuB Search term: AtuB

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000265322 peroxisomal trans-2-enoyl-CoA reductase [Source:HGNC Symbol;Acc:HGNC:18281] E-value: 3.0e-49 Percent Identity: 41.7

References

Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa. Förster-Fromme K, Höschle B, Mack C, Bott M, Armbruster W, Jendrossek D Appl Environ Microbiol 2006 Jul;72(7):4819-28 PubMed ID: 16820476

Structural characterization of the Pseudomonas aeruginosa dehydrogenase AtuB involved in citronellol and geraniol catabolism. Chen Y, Jia H, Liang Y, Zhang H, Che S, Liu R, Zhang Q, Bartlam M Biochem Biophys Res Commun 2020 Mar 19;523(4):954-960 PubMed ID: 31964529