Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3045
|
Name |
rocA2
|
Replicon | chromosome |
Genomic location | 3408560 - 3409183 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Bacterial two hybrid studies in Pseudomonas aeruginosa PAK have shown that RocS1 and RocS2 can both interact with RocA2. Furthermore, a microarray study of PAK and PAK?rocA2, both overexpressing rocS2, revealed that RocA2 represses the expression of the mexAB-oprM gene cluster. qRT-PCR studies confirmed that the same signalling can be achieved by overexpressing rocS1, and in both cases the repression of mexAB-oprM led to an increased sensitivity to antibiotics. |
Comment | RocA2 can interact with two sensors, RocS1 (PA3946) and RocS2 (PA3044). Signalling by RocS1 and RocS2 through RocA2 and leads to repression of the mexAB-oprM gene cluster and increased sensitivity to antibiotics. |
RefSeq | NP_251735.1 |
GI | 15598241 |
Affymetrix | PA3045_at |
DNASU | PaCD00007342 |
Entrez | 882868 |
GenBank | AAG06433.1 |
INSDC | AAG06433.1 |
NCBI Locus Tag | PA3045 |
protein_id(GenBank) | gb|AAG06433.1|AE004729_7|gnl|PseudoCAP|PA3045 |
TIGR | NTL03PA03045 |
UniParc | UPI00000C58E5 |
UniProtKB Acc | Q9HZG3 |
UniProtKB ID | Q9HZG3_PSEAE |
UniRef100 | UniRef100_W0YS37 |
UniRef50 | UniRef50_Q4K750 |
UniRef90 | UniRef90_W0YS37 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Two-component response regulator, RocA2
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -0.56 |
Kyte-Doolittle Hydrophobicity Value | 0.274 |
Molecular Weight (kDa) | 22.5 |
Isoelectric Point (pI) | 6.53 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 616 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000034 (777 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3045
Search term: rocA2
Search term: Two-component response regulator, RocA2
|
Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression.
Sivaneson M, Mikkelsen H, Ventre I, Bordi C, Filloux A
Mol Microbiol 2011 Mar;79(5):1353-66
PubMed ID: 21205015
|