Pseudomonas aeruginosa PAO1, PA3204 (cpxR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3204
Name
cpxR
Replicon chromosome
Genomic location 3594581 - 3595258 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251894.1
GI 15598400
Affymetrix PA3204_at
Entrez 882544
GenBank AAG06592.1
INSDC AAG06592.1
NCBI Locus Tag PA3204
protein_id(GenBank) gb|AAG06592.1|AE004744_7|gnl|PseudoCAP|PA3204
TIGR NTL03PA03204
UniParc UPI00000C594A
UniProtKB Acc Q9HZ36
UniProtKB ID Q9HZ36_PSEAE
UniRef100 UniRef100_W0YUZ4
UniRef50 UniRef50_Q4KGB4
UniRef90 UniRef90_M4WZL9

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component response regulator CpxR
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.62
Kyte-Doolittle Hydrophobicity Value -0.280
Molecular Weight (kDa) 24.8
Isoelectric Point (pI) 5.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 622 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002411 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3204
Search term: cpxR

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_68_C2
Genomic Position 3594906
Position in ORF 326(678)
Reference 15805499
Type ISlacZ/hah insertion site
Mutant ID UWGC: 4716
Genomic Position 3594608
Position in ORF 28(678)
Reference 14617778

Transposon Insertions in Orthologs

References

The cpx proteins of Escherichia coli K12. Structure of the cpxA polypeptide as an inner membrane component.
Weber RF, Silverman PM
J. Mol. Biol. 1988 Sep;203(2):467-78
PubMed ID: 3058985
CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa.
Tian ZX, Yi XX, Cho A, O'Gara F, Wang YP
PLoS Pathog. 2016 Oct;12(10):e1005932
PubMed ID: 27736975