Pseudomonas aeruginosa PAO1, PA3242 (htrB2)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3242
Name	htrB2 Synonym: htrB
Replicon	chromosome
Genomic location	3629667 - 3630605 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_251932.1
GI	15598438
Affymetrix	PA3242_at
DNASU	PaCD00007428
Entrez	882405
GenBank	AAG06630.1
INSDC	AAG06630.1
NCBI Locus Tag	PA3242
protein_id(GenBank)	gb|AAG06630.1|AE004747_3|gnl|PseudoCAP|PA3242
TIGR	NTL03PA03242
UniParc	UPI00000C596D
UniProtKB Acc	Q9HYZ8
UniProtKB ID	Q9HYZ8_PSEAE
UniRef100	UniRef100_Q9HYZ8
UniRef50	UniRef50_Q87YC9
UniRef90	UniRef90_Q02Q73

Product

Feature Type	CDS
Coding Frame	1
Product Name	temperature-regulated acyltransferase HtrB1 Product Name Confidence: Class 1
Synonyms	HtrB2 probable lauroyl acyltransferase
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	14.07
Kyte-Doolittle Hydrophobicity Value	-0.348
Molecular Weight (kDa)	36.1
Isoelectric Point (pI)	10.23

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543 Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 300 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002366 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3242 Search term: htrB2 Search term: temperature-regulated acyltransferase HtrB1

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides. Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET Eur J Biochem 1991 Jun 15;198(3):697-704 PubMed ID: 1904818

Endotoxin biosynthesis in Pseudomonas aeruginosa: enzymatic incorporation of laurate before 3-deoxy-D-manno-octulosonate. Mohan S, Raetz CR J Bacteriol 1994 Nov;176(22):6944-51 PubMed ID: 7961456

Function of the htrB high temperature requirement gene of Escherichia coli in the acylation of lipid A: HtrB catalyzed incorporation of laurate. Clementz T, Bednarski JJ, Raetz CR J Biol Chem 1996 May 17;271(20):12095-102 PubMed ID: 8662613