Pseudomonas aeruginosa PAO1, PA3248

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3248
Name
Replicon	chromosome
Genomic location	3634924 - 3635589 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	4 transposon mutants in orthologs
Comment	Translation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq	NP_251938.1
GI	15598444
Affymetrix	PA3248_at
DNASU	PaCD00007432
Entrez	882411
GenBank	AAG06636.1
INSDC	AAG06636.1
NCBI Locus Tag	PA3248
protein_id(GenBank)	gb|AAG06636.1|AE004747_9|gnl|PseudoCAP|PA3248
TIGR	NTL03PA03248
UniParc	UPI00000C5971
UniProtKB Acc	Q9HYZ2
UniProtKB ID	Q9HYZ2_PSEAE
UniRef100	UniRef100_Q9HYZ2
UniRef50	UniRef50_Q9HYZ2
UniRef90	UniRef90_Q9HYZ2

Product

Feature Type	CDS
Coding Frame	1
Product Name	Uncharacterized protein Product Name Confidence: Class 1
Synonyms	hypothetical protein
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	2.56
Kyte-Doolittle Hydrophobicity Value	-0.251
Molecular Weight (kDa)	20.8
Isoelectric Point (pI)	8.01

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 2 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 275 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001387 (1816 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3248 Search term: Uncharacterized protein

Human Homologs

References

LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames. Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K J Proteomics 2014 Jan 16;96:328-342 PubMed ID: 24291602