Pseudomonas aeruginosa PAO1, PA3485 (tsi3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3485
Name
tsi3
Replicon chromosome
Genomic location 3899501 - 3899938 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252175.1
GI 15598681
Affymetrix PA3485_r_at
DNASU PaCD00007532
Entrez 879938
GenBank AAG06873.1
INSDC AAG06873.1
NCBI Locus Tag PA3485
protein_id(GenBank) gb|AAG06873.1|AE004769_6|gnl|PseudoCAP|PA3485
TIGR NTL03PA03485
UniParc UPI00000C5A33
UniProtKB Acc Q9HYC4
UniProtKB ID Q9HYC4_PSEAE
UniRef100 UniRef100_W0YWF6
UniRef50 UniRef50_A6V1U0
UniRef90 UniRef90_Q02QX4

Product

Feature Type CDS
Coding Frame 1
Product Name
Tsi3
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -3.79
Kyte-Doolittle Hydrophobicity Value -0.443
Molecular Weight (kDa) 15.8
Isoelectric Point (pI) 4.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4M5F HYDROLASE INHIBITOR 08/08/13 complex structure of Tse3-Tsi3 Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0
8IK0 IMMUNE SYSTEM 02/28/23 Cryo-EM structure of Stimulator of interferon genes Gallus gallus; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.3 ELECTRON MICROSCOPY 100.0
4N88 HYDROLASE/HYDROLASE INHIBITOR 10/17/13 Crystal structure of Tse3-Tsi3 complex with calcium ion Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0
4N80 HYDROLASE/HYDROLASE INHIBITOR 10/16/13 Crystal structure of Tse3-Tsi3 complex Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 98.4
8IK3 IMMUNE SYSTEM 02/28/23 Structure of Stimulator of interferon genes/ligand complex Homo sapiens 3.3 ELECTRON MICROSCOPY 100.0
4LUQ PROTEIN BINDING/TOXIN INHIBITOR 07/25/13 Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3 Pseudomonas aeruginosa 1.77 X-RAY DIFFRACTION 98.4
3WA5 HYDROLASE 04/26/13 Crystal Structure of type VI peptidoglycan muramidase effector Tse3 in complex with its cognate immunity protein Tsi3 Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
4N7S HYDROLASE/HYDROLASE INHIBITOR 10/16/13 Crystal structure of Tse3-Tsi3 complex with Zinc ion Pseudomonas aeruginosa 2.101 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002157 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3485
Search term: tsi3
Search term: Tsi3

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Type VI secretion delivers bacteriolytic effectors to target cells.
Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD
Nature 2011 Jul 20;475(7356):343-7
PubMed ID: 21776080