Pseudomonas aeruginosa PAO1, PA4067 (oprG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4067
Name
oprG
Synonym: yciD
Synonym: ompW
Replicon chromosome
Genomic location 4544607 - 4545305 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_252756.1
GI 15599262
Affymetrix PA4067_oprG_at
DNASU PaCD00007777
Entrez 879793
GenBank AAG07454.1
INSDC AAG07454.1
NCBI Locus Tag PA4067
protein_id(GenBank) gb|AAG07454.1|AE004822_14|gnl|PseudoCAP|PA4067
TIGR NTL03PA04067
UniParc UPI00000C5BED
UniProtKB Acc Q9HWW1
UniProtKB ID Q9HWW1_PSEAE
UniRef100 UniRef100_W0YV82
UniRef50 UniRef50_Q88QI8
UniRef90 UniRef90_A6V036

Product

Feature Type CDS
Coding Frame 1
Product Name
Outer membrane protein OprG precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using 2-DE, MALDI-TOF/MS analysis (25.19 kDa, pI 4.8, PMID:16335974)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (25.19 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.29
Kyte-Doolittle Hydrophobicity Value -0.105
Molecular Weight (kDa) 25.2
Isoelectric Point (pI) 4.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2N6P MEMBRANE PROTEIN 08/27/15 Solution NMR structure of Outer Membrane Protein G P92A mutant from Pseudomonas aeruginosa Pseudomonas aeruginosa NOT SOLUTION NMR 99.5
2X27 MEMBRANE PROTEIN 01/11/10 Crystal structure of the outer membrane protein OprG from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
2N6L MEMBRANE PROTEIN 08/25/15 Solution NMR structure of Outer Membrane Protein G from Pseudomonas aeruginosa Pseudomonas aeruginosa NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 148 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001618 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4067
Search term: oprG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Science 1997 Sep 5;277(5331):1453-62
PubMed ID: 9278503
Identification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa.
Gensberg K, Smith AW, Brinkman FS, Hancock RE
J Antimicrob Chemother 1999 Apr;43(4):607-8
PubMed ID: 10350397
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878