Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4067
|
Name |
oprG
Synonym: yciD Synonym: ompW |
Replicon | chromosome |
Genomic location | 4544607 - 4545305 (+ strand) |
Transposon Mutants | 6 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_252756.1 |
GI | 15599262 |
Affymetrix | PA4067_oprG_at |
DNASU | PaCD00007777 |
Entrez | 879793 |
GenBank | AAG07454.1 |
INSDC | AAG07454.1 |
NCBI Locus Tag | PA4067 |
protein_id(GenBank) | gb|AAG07454.1|AE004822_14|gnl|PseudoCAP|PA4067 |
TIGR | NTL03PA04067 |
UniParc | UPI00000C5BED |
UniProtKB Acc | Q9HWW1 |
UniProtKB ID | Q9HWW1_PSEAE |
UniRef100 | UniRef100_W0YV82 |
UniRef50 | UniRef50_Q88QI8 |
UniRef90 | UniRef90_A6V036 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Outer membrane protein OprG precursor
|
Synonyms | |
Evidence for Translation |
Detected using 2-DE, MALDI-TOF/MS analysis (25.19 kDa, pI 4.8, PMID:16335974)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (25.19 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -6.29 |
Kyte-Doolittle Hydrophobicity Value | -0.105 |
Molecular Weight (kDa) | 25.2 |
Isoelectric Point (pI) | 4.68 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2N6P | MEMBRANE PROTEIN | 08/27/15 | Solution NMR structure of Outer Membrane Protein G P92A mutant from Pseudomonas aeruginosa | Pseudomonas aeruginosa | NOT | SOLUTION NMR | 99.5 |
2X27 | MEMBRANE PROTEIN | 01/11/10 | Crystal structure of the outer membrane protein OprG from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 2.4 | X-RAY DIFFRACTION | 100.0 |
2N6L | MEMBRANE PROTEIN | 08/25/15 | Solution NMR structure of Outer Membrane Protein G from Pseudomonas aeruginosa | Pseudomonas aeruginosa | NOT | SOLUTION NMR | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 148 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001618 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4067
Search term: oprG
Search term: Outer membrane protein OprG precursor
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Science 1997 Sep 5;277(5331):1453-62
PubMed ID: 9278503
|
Identification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa.
Gensberg K, Smith AW, Brinkman FS, Hancock RE
J Antimicrob Chemother 1999 Apr;43(4):607-8
PubMed ID: 10350397
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|