Pseudomonas aeruginosa PAO1, PA4491 (magB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4491
Name
magB
Synonym: yfaA
Synonym: pufY
Replicon chromosome
Genomic location 5025614 - 5027383 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253181.1
GI 15599687
Affymetrix PA4491_at
Entrez 881054
GenBank AAG07879.1
INSDC AAG07879.1
NCBI Locus Tag PA4491
protein_id(GenBank) gb|AAG07879.1|AE004862_13|gnl|PseudoCAP|PA4491
TIGR NTL03PA04492
UniParc UPI00000C5D33
UniProtKB Acc Q9HVT0
UniProtKB ID Q9HVT0_PSEAE
UniRef100 UniRef100_Q9HVT0
UniRef50 UniRef50_Q9HVT0
UniRef90 UniRef90_Q9HVT0

Product

Feature Type CDS
Coding Frame 1
Product Name
MagB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (64.21 kDa, PMID:21751344)
Charge (pH 7) -12.82
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 64.2
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4251
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 9 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004079 (359 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4491
Search term: magB
Search term: MagB

Human Homologs

References

The parD- mutant of Escherichia coli also carries a gyrAam mutation. The complete sequence of gyrA.
Hussain K, Elliott EJ, Salmond GP
Mol Microbiol 1987 Nov;1(3):259-73
PubMed ID: 2834621
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Unique features of a Pseudomonas aeruginosa α2-macroglobulin homolog.
Robert-Genthon M, Casabona MG, Neves D, Couté Y, Cicéron F, Elsen S, Dessen A, Attrée I
MBio 2013 Aug;4(4):
PubMed ID: 23919994