Pseudomonas aeruginosa PAO1, PA4650 (cupE3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4650
Name
cupE3
Replicon chromosome
Genomic location 5216203 - 5216745 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentThe cupE gene cluster encodes a chaperone usher pathway that is homologous to the A. baumanii csu gene cluster. Activation of the cupE genes leads to the production and assembly of CupE fimbriae on the cell surface. These fimbriae are important in early and late stages of biofilm formation. The expression of the cupE gene cluster is controlled by the two-component system PprA-PprB, and binding of PprB to the cupE1 promoter has been confirmed by EMSA.

Cross-References

RefSeq NP_253339.1
GI 15599845
Affymetrix PA4650_at
DNASU PaCD00008017
Entrez 881294
GenBank AAG08037.1
INSDC AAG08037.1
NCBI Locus Tag PA4650
protein_id(GenBank) gb|AAG08037.1|AE004879_3|gnl|PseudoCAP|PA4650
TIGR NTL03PA04651
UniParc UPI00000C5DAB
UniProtKB Acc Q9HVE2
UniProtKB ID Q9HVE2_PSEAE
UniRef100 UniRef100_W0Z1C6
UniRef50 UniRef50_A0A089V5R6
UniRef90 UniRef90_W1MNL1

Product

Feature Type CDS
Coding Frame 1
Product Name
Pilin subunit CupE3
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 3.15
Kyte-Doolittle Hydrophobicity Value 0.261
Molecular Weight (kDa) 18.8
Isoelectric Point (pI) 9.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 47 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004301 (613 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4650
Search term: cupE3

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The PprA-PprB two-component system activates CupE, the first non-archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling fimbriae.
Giraud C, Bernard CS, Calderon V, Yang L, Filloux A, Molin S, Fichant G, Bordi C, de Bentzmann S
Environ Microbiol 2011 Mar;13(3):666-83
PubMed ID: 21091863