Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4661
|
Name |
pagL
|
Replicon | chromosome |
Genomic location | 5229459 - 5229980 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253350.1 |
GI | 15599856 |
Affymetrix | PA4661_at |
Entrez | 881344 |
GenBank | AAG08048.1 |
INSDC | AAG08048.1 |
NCBI Locus Tag | PA4661 |
protein_id(GenBank) | gb|AAG08048.1|AE004880_4|gnl|PseudoCAP|PA4661 |
TIGR | NTL03PA04662 |
UniParc | UPI00000C5DB5 |
UniProtKB Acc | Q9HVD1 |
UniProtKB ID | PAGL_PSEAE |
UniRef100 | UniRef100_Q9HVD1 |
UniRef50 | UniRef50_Q9HVD1 |
UniRef90 | UniRef90_Q9HVD1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Lipid A 3-O-deacylase
|
Synonyms | |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (18.39 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using 2-DE, MALDI-TOF/MS analysis (18.39 kDa, pI 5.9, PMID:16335974)
|
Charge (pH 7) | -1.30 |
Kyte-Doolittle Hydrophobicity Value | -0.042 |
Molecular Weight (kDa) | 18.4 |
Isoelectric Point (pI) | 6.25 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2ERV | MEMBRANE PROTEIN | 10/25/05 | Crystal structure of the outer membrane enzyme PagL | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 31 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004311 (531 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4661
Search term: pagL
Search term: Lipid A 3-O-deacylase
|
Dissemination of lipid A deacylases (pagL) among gram-negative bacteria: identification of active-site histidine and serine residues.
Geurtsen J, Steeghs L, Hove JT, van der Ley P, Tommassen J
J Biol Chem 2005 Mar 4;280(9):8248-59
PubMed ID: 15611102
|
The Pseudomonas aeruginosa lipid A deacylase: selection for expression and loss within the cystic fibrosis airway.
Ernst RK, Adams KN, Moskowitz SM, Kraig GM, Kawasaki K, Stead CM, Trent MS, Miller SI
J Bacteriol 2006 Jan;188(1):191-201
PubMed ID: 16352835
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|