Pseudomonas aeruginosa PAO1, PA4675 (chtA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4675
Name	chtA Synonym: optH Synonym: iutA
Replicon	chromosome
Genomic location	5243178 - 5245406 (+ strand)
Transposon Mutants	6 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq	NP_253364.1
GI	15599870
Affymetrix	PA4675_at
Entrez	881415
GenBank	AAG08062.1
INSDC	AAG08062.1
NCBI Locus Tag	PA4675
protein_id(GenBank)	gb|AAG08062.1|AE004881_5|gnl|PseudoCAP|PA4675
TIGR	NTL03PA04676
UniParc	UPI00000C5DBB
UniProtKB Acc	Q9HVC0
UniProtKB ID	Q9HVC0_PSEAE
UniRef100	UniRef100_W0Z0E3
UniRef50	UniRef50_K0CAU9
UniRef90	UniRef90_V6AMN6

Product

Feature Type	CDS
Coding Frame	1
Product Name	ChtA Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Detected using LC-MS/MS (80.95 kDa, PMID:21751344) Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-10.82
Kyte-Doolittle Hydrophobicity Value	-0.401
Molecular Weight (kDa)	81.0
Isoelectric Point (pI)	5.21

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Vesicle Class 1 Outer Membrane Class 2 Outer Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 285 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004325 (382 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4675 Search term: chtA Search term: ChtA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Identification and characterization of an iron-regulated gene, chtA, required for the utilization of the xenosiderophores aerobactin, rhizobactin 1021 and schizokinen by Pseudomonas aeruginosa. Cuív PÓ, Clarke P, O'Connell M Microbiology (Reading) 2006 Apr;152(Pt 4):945-954 PubMed ID: 16549659

Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix. Toyofuku M, Roschitzki B, Riedel K, Eberl L J Proteome Res 2012 Oct 5;11(10):4906-15 PubMed ID: 22909304

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms. Couto N, Schooling SR, Dutcher JR, Barber J J Proteome Res 2015 Oct 2;14(10):4207-22 PubMed ID: 26303878