Pseudomonas aeruginosa PAO1, PA4676

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4676
Name
Synonym: yadF
Replicon chromosome
Genomic location 5245476 - 5246123 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253365.1
GI 15599871
Affymetrix PA4676_at
DNASU PaCD00008031
Entrez 881416
GenBank AAG08063.1
INSDC AAG08063.1
NCBI Locus Tag PA4676
protein_id(GenBank) gb|AAG08063.1|AE004881_6|gnl|PseudoCAP|PA4676
TIGR NTL03PA04677
UniParc UPI00000C5DBC
UniProtKB Acc Q9HVB9
UniProtKB ID Q9HVB9_PSEAE
UniRef100 UniRef100_W0Z3D7
UniRef50 UniRef50_W0Z3D7
UniRef90 UniRef90_W0Z3D7

Product

Feature Type CDS
Coding Frame 1
Product Name
probable carbonic anhydrase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.06
Kyte-Doolittle Hydrophobicity Value -0.149
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 6.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5BQ1 LYASE 05/28/15 Capturing Carbon Dioxide in beta Carbonic Anhydrase PSEUDOMONAS AERUGINOSA 1.6 X-RAY DIFFRACTION 100.0
4RXY LYASE 12/12/14 Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.901 X-RAY DIFFRACTION 100.0
5JJ8 LYASE 04/22/16 Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa - alternate crystal packing form PSEUDOMONAS AERUGINOSA 2.585 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 387 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004326 (497 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4676

References

Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region.
Fujita N, Mori H, Yura T, Ishihama A
Nucleic Acids Res. 1994 May;22(9):1637-9
PubMed ID: 8202364
Amino acid sequence homology between N- and C-terminal halves of a carbonic anhydrase in Porphyridium purpureum, as deduced from the cloned cDNA.
Mitsuhashi S, Miyachi S
J. Biol. Chem. 1996 Nov;271(45):28703-9
PubMed ID: 8910506
Three functional β-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air.
Lotlikar SR, Hnatusko S, Dickenson NE, Choudhari SP, Picking WL, Patrauchan MA
Microbiology (Reading, Engl.) 2013 Aug;159(Pt 8):1748-59
PubMed ID: 23728627