Pseudomonas aeruginosa PAO1, PA4676 (psCA3)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4676
Name	psCA3 Synonym: yadF
Replicon	chromosome
Genomic location	5245476 - 5246123 (- strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_253365.1
GI	15599871
Affymetrix	PA4676_at
DNASU	PaCD00008031
Entrez	881416
GenBank	AAG08063.1
INSDC	AAG08063.1
NCBI Locus Tag	PA4676
protein_id(GenBank)	gb|AAG08063.1|AE004881_6|gnl|PseudoCAP|PA4676
TIGR	NTL03PA04677
UniParc	UPI00000C5DBC
UniProtKB Acc	Q9HVB9
UniProtKB ID	Q9HVB9_PSEAE
UniRef100	UniRef100_W0Z3D7
UniRef50	UniRef50_W0Z3D7
UniRef90	UniRef90_W0Z3D7

Product

Feature Type	CDS
Coding Frame	1
Product Name	beta-carbonic anhydrase Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-5.06
Kyte-Doolittle Hydrophobicity Value	-0.149
Molecular Weight (kDa)	24.2
Isoelectric Point (pI)	6.01

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5BQ1	LYASE	05/28/15	Capturing Carbon Dioxide in beta Carbonic Anhydrase	Pseudomonas aeruginosa	1.6	X-RAY DIFFRACTION	100.0
6D2N	LYASE	04/13/18	Beta Carbonic anhydrase in complex with a sulfonamide anion	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	100.0
6D2O	LYASE	04/13/18	Beta Carbonic anhydrase in complex with 4-methylimidazole	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	100.0
4RXY	LYASE	12/12/14	Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa	Pseudomonas aeruginosa	1.901	X-RAY DIFFRACTION	100.0
5JJ8	LYASE	04/22/16	Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa - alternate crystal packing form	Pseudomonas aeruginosa	2.585	X-RAY DIFFRACTION	100.0
6D2M	LYASE	04/13/18	Beta Carbonic anhydrase in complex with thiocyanate	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	100.0
6D2J	LYASE	04/13/18	Beta Carbonic anhydrase in complex with thiocyanate	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 387 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004326 (477 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4676 Search term: psCA3 Search term: beta-carbonic anhydrase

Human Homologs

References

Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region. Fujita N, Mori H, Yura T, Ishihama A Nucleic Acids Res 1994 May 11;22(9):1637-9 PubMed ID: 8202364

Amino acid sequence homology between N- and C-terminal halves of a carbonic anhydrase in Porphyridium purpureum, as deduced from the cloned cDNA. Mitsuhashi S, Miyachi S J Biol Chem 1996 Nov 8;271(45):28703-9 PubMed ID: 8910506

Three functional β-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Lotlikar SR, Hnatusko S, Dickenson NE, Choudhari SP, Picking WL, Patrauchan MA Microbiology (Reading, Engl.) 2013 Aug;159(Pt 8):1748-59 PubMed ID: 23728627

Structural Mapping of Anion Inhibitors to β-Carbonic Anhydrase psCA3 from Pseudomonas aeruginosa. Murray AB, Aggarwal M, Pinard M, Vullo D, Patrauchan M, Supuran CT, McKenna R ChemMedChem 2018 Oct 8;13(19):2024-2029 PubMed ID: 30088334

Structure and inhibition studies of a type II beta-carbonic anhydrase psCA3 from Pseudomonas aeruginosa. Pinard MA, Lotlikar SR, Boone CD, Vullo D, Supuran CT, Patrauchan MA, McKenna R Bioorg Med Chem 2015 Aug 1;23(15):4831-4838 PubMed ID: 26068018