Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4781
|
Name |
|
Replicon | chromosome |
Genomic location | 5369187 - 5370368 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_253469.1 |
GI | 15599975 |
Affymetrix | PA4781_at |
Entrez | 880075 |
GenBank | AAG08167.1 |
INSDC | AAG08167.1 |
NCBI Locus Tag | PA4781 |
protein_id(GenBank) | gb|AAG08167.1|AE004891_9|gnl|PseudoCAP|PA4781 |
TIGR | NTL03PA04782 |
UniParc | UPI00000C5E00 |
UniProtKB Acc | Q9HV27 |
UniProtKB ID | Q9HV27_PSEAE |
UniRef100 | UniRef100_W0YXL1 |
UniRef50 | UniRef50_A6VCP0 |
UniRef90 | UniRef90_A6VCP0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
cyclic di-GMP phosphodiesterase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -12.84 |
Kyte-Doolittle Hydrophobicity Value | -0.267 |
Molecular Weight (kDa) | 43.6 |
Isoelectric Point (pI) | 4.98 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4R8Z | HYDROLASE | 09/03/14 | Crystal Structure of PA4781 HD-GYP domain from Pseudomonas aeruginosa at 2.2A resolution showing a bi-metallic Ni ion center | Pseudomonas aeruginosa PAO1 | 2.2 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | PseudoCAP |
Category | |
Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
Host Organism | Galleria mellonella |
Notes | |
Infection model | Galleria mellonella killing assay |
Reference | 19170727 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002973 (763 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4781
Search term: cyclic di-GMP phosphodiesterase
|
HD-GYP domain proteins regulate biofilm formation and virulence in Pseudomonas aeruginosa.
Ryan RP, Lucey J, O'Donovan K, McCarthy Y, Yang L, Tolker-Nielsen T, Dow JM
Environ Microbiol 2009 May;11(5):1126-36
PubMed ID: 19170727
|