Pseudomonas aeruginosa PAO1, PA4781

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4781
Name
Replicon chromosome
Genomic location 5369187 - 5370368 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253469.1
GI 15599975
Affymetrix PA4781_at
Entrez 880075
GenBank AAG08167.1
INSDC AAG08167.1
NCBI Locus Tag PA4781
protein_id(GenBank) gb|AAG08167.1|AE004891_9|gnl|PseudoCAP|PA4781
TIGR NTL03PA04782
UniParc UPI00000C5E00
UniProtKB Acc Q9HV27
UniProtKB ID Q9HV27_PSEAE
UniRef100 UniRef100_W0YXL1
UniRef50 UniRef50_A6VCP0
UniRef90 UniRef90_A6VCP0

Product

Feature Type CDS
Coding Frame 1
Product Name
cyclic di-GMP phosphodiesterase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.84
Kyte-Doolittle Hydrophobicity Value -0.267
Molecular Weight (kDa) 43.6
Isoelectric Point (pI) 4.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R8Z HYDROLASE 09/03/14 Crystal Structure of PA4781 HD-GYP domain from Pseudomonas aeruginosa at 2.2A resolution showing a bi-metallic Ni ion center Pseudomonas aeruginosa PAO1 2.2 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Galleria mellonella
Notes
Infection model Galleria mellonella killing assay
Reference 19170727

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002973 (763 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4781

Human Homologs

References

HD-GYP domain proteins regulate biofilm formation and virulence in Pseudomonas aeruginosa.
Ryan RP, Lucey J, O'Donovan K, McCarthy Y, Yang L, Tolker-Nielsen T, Dow JM
Environ Microbiol 2009 May;11(5):1126-36
PubMed ID: 19170727