Pseudomonas aeruginosa PAO1, PA5365 (phoU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5365
Name
phoU
Replicon chromosome
Genomic location 6032387 - 6033115 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254052.1
GI 15600558
Affymetrix PA5365_phoU_at
Entrez 881627
GenBank AAG08750.1
INSDC AAG08750.1
NCBI Locus Tag PA5365
protein_id(GenBank) gb|AAG08750.1|AE004948_6|gnl|PseudoCAP|PA5365
TIGR NTL03PA05366
UniParc UPI00001319DA
UniProtKB Acc Q51547
UniProtKB ID PHOU_PSEAE
UniRef100 UniRef100_Q51547
UniRef50 UniRef50_Q51547
UniRef90 UniRef90_Q51547

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphate uptake regulatory protein PhoU
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.61
Kyte-Doolittle Hydrophobicity Value -0.347
Molecular Weight (kDa) 27.5
Isoelectric Point (pI) 4.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4Q25 UNKNOWN FUNCTION 04/07/14 Crystal structure of PhoU from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.28 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 523 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004930 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5365
Search term: phoU

Human Homologs

References

Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis.
Kato J, Sakai Y, Nikata T, Ohtake H
J Bacteriol 1994 Sep;176(18):5874-7
PubMed ID: 8083184
Molecular analysis of the phosphate-specific transport (pst) operon of Pseudomonas aeruginosa.
Nikata T, Sakai Y, Shibat K, Kato J, Kuroda A, Ohtake H
Mol Gen Genet 1996 Apr 10;250(6):692-8
PubMed ID: 8628229