Pseudomonas aeruginosa UCBPP-PA14, PA14_19490 (lsfA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_19490
Name
lsfA
Replicon chromosome
Genomic location 1684916 - 1685554 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_789697.1
GI 116051470
Entrez 4385236
INSDC ABJ12706.1
NCBI Locus Tag PA14_19490
UniParc UPI00003960B2
UniProtKB Acc Q02QT9
UniProtKB ID Q02QT9_PSEAB
UniRef100 UniRef100_A0A071KYB2
UniRef50 UniRef50_Q54SE2
UniRef90 UniRef90_A0A024HLA3

Product

Feature Type CDS
Coding Frame 1
Product Name
1-Cys peroxiredoxin LsfA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -5.83
Kyte-Doolittle Hydrophobicity Value -0.423
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category 1-Cys peroxyredoxin
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes Mice infected with lsfA C45A mutant strain showed a reduced bacterial burden in all organs analyzed compared with wild-type PA14-infected
Infection model in vitro infection of J774 macrophages
Infection model Mouse acute pneumonia model (host
Reference 25329795

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002190 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_19490
Search term: lsfA

Human Homologs

Ensembl 86, assembly GRCh38.p7
peroxiredoxin 6 [Source:HGNC Symbol;Acc:HGNC:16753]
E-value: 1.1e-59
Percent Identity: 52.9

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 43753
Genomic Position 1685165
Position in ORF 250(639)
Reference 16477005

Transposon Insertions in Orthologs

Type Confirmed ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA3450
Mutant ID UWGC: 5239
Genomic Position 3855959
Position in ORF 467(639)
Reference 14617778
Type ISlacZ/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA3450
Mutant ID UWGC: 19470
Genomic Position 3855754
Position in ORF 262(639)
Reference 14617778

References

No references are associated with this feature.