Pseudomonas aeruginosa PAO1, PA1685 (masA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1685
Name
masA
Replicon chromosome
Genomic location 1834393 - 1835142 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_250376.1
GI 15596882
Affymetrix PA1685_masA_at
Entrez 878111
GenBank AAG05074.1
INSDC AAG05074.1
NCBI Locus Tag PA1685
protein_id(GenBank) gb|AAG05074.1|AE004595_13|gnl|PseudoCAP|PA1685
TIGR NTL03PA01686
UniParc UPI00000C5446
UniProtKB Acc Q9I340
UniProtKB ID MTNC_PSEAE
UniRef100 UniRef100_Q9I340
UniRef50 UniRef50_Q48KM6
UniRef90 UniRef90_Q9I340

Product

Feature Type CDS
Coding Frame 1
Product Name
enolase-phosphatase E-1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.07
Kyte-Doolittle Hydrophobicity Value -0.003
Molecular Weight (kDa) 26.5
Isoelectric Point (pI) 4.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 74 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003705 (401 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1685
Search term: masA

Human Homologs

Ensembl 110, assembly GRCh38.p14
enolase-phosphatase 1 [Source:HGNC Symbol;Acc:HGNC:24599]
E-value: 1.1e-36
Percent Identity: 37.6

References

Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae.
Myers RW, Wray JW, Fish S, Abeles RH
J Biol Chem 1993 Nov 25;268(33):24785-91
PubMed ID: 8227039
Appendix. Cloning and sequence of the gene encoding enzyme E-1 from the methionine salvage pathway of Klebsiella oxytoca.
Balakrishnan R, Frohlich M, Rahaim PT, Backman K, Yocum RR
J Biol Chem 1993 Nov 25;268(33):24792-5
PubMed ID: 8227040