Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3947
|
Name |
rocR
Synonym: rocR |
Replicon | chromosome |
Genomic location | 4426952 - 4428130 (- strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
Comment | Genetic and bacterial two hybrid studies in Pseudomonas aeruginosa PAK have revealed that RocS1-induced expression of the cupB and cupC gene clusters is repressed by RocR. Furthermore, purified RocR has been shown to display phosphodiesterase activity and degrades the second messenger c-di-GMP. |
Comment | Overexpression of PA3947 impairs TTSS-mediated cytotoxicity |
Comment | RocR has been shown to have c-di-GMP phosphodiesterase activity and to repress the expression of the cupB and cupC gene clusters. |
RefSeq | NP_252636.1 |
GI | 15599142 |
Affymetrix | PA3947_at |
DNASU | PaCD00007723 |
Entrez | 878871 |
GenBank | AAG07334.1 |
INSDC | AAG07334.1 |
NCBI Locus Tag | PA3947 |
protein_id(GenBank) | gb|AAG07334.1|AE004813_1|gnl|PseudoCAP|PA3947 |
TIGR | NTL03PA03947 |
UniParc | UPI00000C5B8F |
UniProtKB Acc | Q9HX69 |
UniProtKB ID | Q9HX69_PSEAE |
UniRef100 | UniRef100_W0YX99 |
UniRef50 | UniRef50_W0YX99 |
UniRef90 | UniRef90_W0YX99 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
RocR
|
Synonyms |
RocR |
Evidence for Translation | |
Charge (pH 7) | -10.07 |
Kyte-Doolittle Hydrophobicity Value | 0.194 |
Molecular Weight (kDa) | 42.8 |
Isoelectric Point (pI) | 5.11 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3SY8 | TRANSCRIPTION REGULATOR | 07/16/11 | Crystal structure of the response regulator RocR | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 99.7 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 405 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001731 (376 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3947
Search term: rocR
Search term: RocR
|
A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes.
Kulasekara HD, Ventre I, Kulasekara BR, Lazdunski A, Filloux A, Lory S
Mol Microbiol 2005 Jan;55(2):368-80
PubMed ID: 15659157
|
Catalytic mechanism of cyclic di-GMP-specific phosphodiesterase: a study of the EAL domain-containing RocR from Pseudomonas aeruginosa.
Rao F, Yang Y, Qi Y, Liang ZX
J Bacteriol 2008 May;190(10):3622-31
PubMed ID: 18344366
|
Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression.
Sivaneson M, Mikkelsen H, Ventre I, Bordi C, Filloux A
Mol Microbiol 2011 Mar;79(5):1353-66
PubMed ID: 21205015
|
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Kulasakara H, Lee V, Brencic A, Liberati N, Urbach J, Miyata S, Lee DG, Neely AN, Hyodo M, Hayakawa Y, Ausubel FM, Lory S
Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2839-44
PubMed ID: 16477007
|