Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4175
|
Name |
piv
Synonym: prpL Synonym: piv |
Replicon | chromosome |
Genomic location | 4671319 - 4672707 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | protease IV is an ocular virulence factor (rabbit model). |
RefSeq | NP_252864.1 |
GI | 15599370 |
Affymetrix | PA4175_at |
Entrez | 880208 |
GenBank | AAG07562.1 |
INSDC | AAG07562.1 |
NCBI Locus Tag | PA4175 |
protein_id(GenBank) | gb|AAG07562.1|AE004834_2|gnl|PseudoCAP|PA4175 |
TIGR | NTL03PA04175 |
UniParc | UPI00000C5C54 |
UniProtKB Acc | Q9HWK6 |
UniProtKB ID | LYSC_PSEAE |
UniRef100 | UniRef100_Q9HWK6 |
UniRef50 | UniRef50_Q9HWK6 |
UniRef90 | UniRef90_Q9HWK6 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
protease IV
|
Synonyms |
Pvds-regulated endoprotease, lysyl class protease IV probable endoproteinase Arg-C precursor |
Evidence for Translation | |
Charge (pH 7) | -0.34 |
Kyte-Doolittle Hydrophobicity Value | -0.097 |
Molecular Weight (kDa) | 48.2 |
Isoelectric Point (pI) | 6.94 |
Individual Mappings | |
Additional evidence for subcellular localization |
Source 1 | |
Database | VFDB |
Category | Protease IV |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 24 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001524 (296 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4175
Search term: piv
Search term: protease IV
|
Characterization of an endoprotease (PrpL) encoded by a PvdS-regulated gene in Pseudomonas aeruginosa.
Wilderman PJ, Vasil AI, Johnson Z, Wilson MJ, Cunliffe HE, Lamont IL, Vasil ML
Infect Immun 2001 Sep;69(9):5385-94
PubMed ID: 11500408
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cloning of a Lysobacter enzymogenes gene that encodes an arginyl endopeptidase (endoproteinase Arg-C).
Wright DS, Graham LD, Jennings PA
Biochim Biophys Acta 1998 Dec 22;1443(3):369-74
PubMed ID: 9878833
|
Pseudomonas keratitis. The role of an uncharacterized exoprotein, protease IV, in corneal virulence.
O'Callaghan RJ, Engel LS, Hobden JA, Callegan MC, Green LC, Hill JM
Invest Ophthalmol Vis Sci 1996 Mar;37(4):534-43
PubMed ID: 8595953
|
Pseudomonas deficient in protease IV has significantly reduced corneal virulence.
Engel LS, Hobden JA, Moreau JM, Callegan MC, Hill JM, O'Callaghan RJ
Invest Ophthalmol Vis Sci 1997 Jul;38(8):1535-42
PubMed ID: 9224281
|
Pseudomonas aeruginosa protease IV produces corneal damage and contributes to bacterial virulence.
Engel LS, Hill JM, Moreau JM, Green LC, Hobden JA, O'Callaghan RJ
Invest Ophthalmol Vis Sci 1998 Mar;39(3):662-5
PubMed ID: 9501882
|
Protease IV, a unique extracellular protease and virulence factor from Pseudomonas aeruginosa.
Engel LS, Hill JM, Caballero AR, Green LC, O'Callaghan RJ
J Biol Chem 1998 Jul 3;273(27):16792-7
PubMed ID: 9642237
|
Pseudomonas aeruginosa protease IV enzyme assays and comparison to other Pseudomonas proteases.
Caballero AR, Moreau JM, Engel LS, Marquart ME, Hill JM, O'Callaghan RJ
Anal Biochem 2001 Mar;290(2):330-7
PubMed ID: 11237336
|
Identification of the active site residues of Pseudomonas aeruginosa protease IV. Importance of enzyme activity in autoprocessing and activation.
Traidej M, Marquart ME, Caballero AR, Thibodeaux BA, O'Callaghan RJ
J Biol Chem 2003 Jan 24;278(4):2549-53
PubMed ID: 12419815
|
Pseudomonas keratitis: protease IV gene conservation, distribution, and production relative to virulence and other Pseudomonas proteases.
Caballero A, Thibodeaux B, Marquart M, Traidej M, O'Callaghan R
Invest Ophthalmol Vis Sci 2004 Feb;45(2):522-30
PubMed ID: 14744894
|
Molecular analysis of Pseudomonas aeruginosa protease IV expressed in Pseudomonas putida.
Traidej M, Caballero AR, Marquart ME, Thibodeaux BA, O'Callaghan RJ
Invest Ophthalmol Vis Sci 2003 Jan;44(1):190-6
PubMed ID: 12506074
|
Calcium and magnesium enhance the production of Pseudomonas aeruginosa protease IV, a corneal virulence factor.
Marquart ME, Dajcs JJ, Caballero AR, Thibodeaux BA, O'Callaghan RJ
Med Microbiol Immunol 2005 Jan;194(1-2):39-45
PubMed ID: 14628143
|
Pseudomonas aeruginosa protease IV: a corneal virulence factor of low immunogenicity.
Thibodeaux BA, Caballero AR, Dajcs JJ, Marquart ME, Engel LS, O'Callaghan RJ
Ocul Immunol Inflamm 2005 Apr-Jun;13(2-3):169-82
PubMed ID: 16019676
|
Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions.
Malloy JL, Veldhuizen RA, Thibodeaux BA, O'Callaghan RJ, Wright JR
Am J Physiol Lung Cell Mol Physiol 2005 Feb;288(2):L409-18
PubMed ID: 15516485
|
Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene.
Fox A, Haas D, Reimmann C, Heeb S, Filloux A, Voulhoux R
Appl Environ Microbiol 2008 Mar;74(6):1902-8
PubMed ID: 18203852
|
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299
|