Pseudomonas aeruginosa UCBPP-PA14, PA14_01110 (vgrG1a)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest |
| Locus Tag |
PA14_01110
|
| Name |
vgrG1a
|
| Replicon | chromosome |
| Genomic location | 108629 - 110560 (+ strand) |
| Transposon Mutants | 1 transposon mutants in UCBPP-PA14 |
| Transposon Mutants in orthologs | 48 transposon mutants in orthologs |
| Comment | Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS) |
Cross-References
| RefSeq | YP_788248.1 |
| GI | 116053811 |
| Entrez | 4383509 |
| NCBI Locus Tag | PA14_01110 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
VgrG1a
|
| Synonyms |
hypothetical protein |
| Evidence for Translation | |
| Charge (pH 7) | -7.09 |
| Kyte-Doolittle Hydrophobicity Value | -0.538 |
| Molecular Weight (kDa) | 72041.1 |
| Isoelectric Point (pI) | 6.28 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4UHV | STRUCTURAL PROTEIN | 03/25/15 | The structure of VgrG1, the needle tip of the bacterial Type VI Secretion System | PSEUDOMONAS AERUGINOSA | 2 | X-RAY DIFFRACTION | 99.5 |
| 4MTK | TOXIN | 09/19/13 | Crystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion System | Pseudomonas aeruginosa | 3.322 | X-RAY DIFFRACTION | 99.5 |
| 6H3N | TOXIN | 07/19/18 | Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs | Pseudomonas aeruginosa PAO1 | 3.25 | ELECTRON MICROSCOPY | 99.5 |
| 6H3L | TOXIN | 07/19/18 | Structure of VgrG1 in the Type VI secretion pre-firing VgrG1-Tse6-EagT6-EF-Tu-Tsi6 complex | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 4.2 | ELECTRON MICROSCOPY | 99.5 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 156 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000090 (3342 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA14_01110
Search term: vgrG1a
Search term: VgrG1a
|
Human Homologs
References
|
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jové D, Filloux A
J Biol Chem 2011 Apr 8;286(14):12317-27
PubMed ID: 21325275
|
|
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999
|