Pseudomonas aeruginosa UCBPP-PA14, PA14_01110 (vgrG1a)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa UCBPP-PA14 - Assembly GCF_000014625.1
GCF_000014625.1|latest |
| Locus Tag |
PA14_01110
|
| Name |
vgrG1a
|
| Replicon | chromosome |
| Genomic location | 108629 - 110560 (+ strand) |
| Transposon Mutants | 1 transposon mutants in UCBPP-PA14 |
| Transposon Mutants in orthologs | 48 transposon mutants in orthologs |
| Comment | Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS) |
Cross-References
| RefSeq | YP_788248.1 |
| GI | 116053811 |
| Entrez | 4383509 |
| NCBI Locus Tag | PA14_01110 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
VgrG1a
|
| Synonyms |
hypothetical protein |
| Evidence for Translation | |
| Charge (pH 7) | -7.09 |
| Kyte-Doolittle Hydrophobicity Value | -0.538 |
| Molecular Weight (kDa) | 72041.1 |
| Isoelectric Point (pI) | 6.28 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4MTK | TOXIN | 09/19/13 | Crystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion System | Pseudomonas aeruginosa | 3.322 | X-RAY DIFFRACTION | 99.5 |
| 6H3L | TOXIN | 07/19/18 | Structure of VgrG1 in the Type VI secretion pre-firing VgrG1-Tse6-EagT6-EF-Tu-Tsi6 complex | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 4.2 | ELECTRON MICROSCOPY | 99.5 |
| 6H3N | TOXIN | 07/19/18 | Structure of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs | Pseudomonas aeruginosa PAO1 | 3.25 | ELECTRON MICROSCOPY | 99.5 |
| 4UHV | STRUCTURAL PROTEIN | 03/25/15 | The structure of VgrG1, the needle tip of the bacterial Type VI Secretion System | PSEUDOMONAS AERUGINOSA | 2 | X-RAY DIFFRACTION | 99.5 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 156 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000090 (3404 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA14_01110
Search term: vgrG1a
Search term: VgrG1a
|
Human Homologs
References
|
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jové D, Filloux A
J. Biol. Chem. 2011 Apr;286(14):12317-27
PubMed ID: 21325275
|
|
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
|