Pseudomonas aeruginosa UCBPP-PA14, PA14_25195 (nagZ)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_25195
Name	nagZ
Replicon	chromosome
Genomic location	2202589 - 2203587 (+ strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	YP_790167.1
GI	116054412
EC Number	3.2.1.52
Entrez	4384185
INSDC	ABJ15648.1
NCBI Locus Tag	PA14_25195
UniParc	UPI000012FD11
UniProtKB Acc	Q02PG9
UniProtKB ID	NAGZ_PSEAB
UniRef100	UniRef100_B7UYS5
UniRef50	UniRef50_Q8ZFS3
UniRef90	UniRef90_B7UYS5

Product

Feature Type	CDS
Coding Frame	1
Product Name	beta-hexosaminidase
Synonyms
Evidence for Translation
Charge (pH 7)	-4.70
Kyte-Doolittle Hydrophobicity Value	-0.068
Molecular Weight (kDa)	36.1
Isoelectric Point (pI)	6.18

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5G1M	HYDROLASE	03/28/16	Crystal structure of NagZ from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	1.8	X-RAY DIFFRACTION	100.0
5G3R	HYDROLASE	04/30/16	Crystal structure of NagZ from Pseudomonas aeruginosa in complex with N-acetylglucosamine and L-Ala-1,6-anhydroMurNAc	PSEUDOMONAS AERUGINOSA	2.18	X-RAY DIFFRACTION	100.0
5LY7	GENE REGULATION	09/25/16	Crystal structure of NagZ H174A mutant from Pseudomonas aeruginosa in complex with the inhibitor 2-acetamido-1,2-dideoxynojirimycin	Pseudomonas aeruginosa	3.1	X-RAY DIFFRACTION	99.7
5G5U	HYDROLASE	06/05/16	Crystal structure of NagZ H174A mutant from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	2.25	X-RAY DIFFRACTION	99.7
5G5K	HYDROLASE	05/25/16	Crystal structure of NagZ from Pseudomonas aeruginosa in complex with the inhibitor 2-acetamido-1,2-dideoxynojirimycin	PSEUDOMONAS AERUGINOSA	3.1	X-RAY DIFFRACTION	100.0
5G6T	HYDROLASE	07/15/16	Crystal structure of Zn-containing NagZ H174A mutant from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	2.15	X-RAY DIFFRACTION	99.7
5G2M	HYDROLASE	04/09/16	Crystal structure of NagZ from Pseudomonas aeruginosa in complex with N-acetylglucosamine	PSEUDOMONAS AERUGINOSA	3	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 482 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002577 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_25195 Search term: nagZ Search term: beta-hexosaminidase

Human Homologs

References