Pseudomonas aeruginosa UCBPP-PA14, PA14_65320 (miaA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_65320
Name	miaA
Replicon	chromosome
Genomic location	5820141 - 5821112 (- strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	YP_793413.1
GI	116053094
EC Number	2.5.1.75
Entrez	4384848
INSDC	ABJ14330.1
NCBI Locus Tag	PA14_65320
UniParc	UPI0000E44C46
UniProtKB Acc	Q02F73
UniProtKB ID	MIAA_PSEAB
UniRef100	UniRef100_Q02F73
UniRef50	UniRef50_A4VQP1
UniRef90	UniRef90_A6VD58

Product

Feature Type	CDS
Coding Frame	1
Product Name	tRNA delta(2)-isopentenylpyrophosphate transferase
Synonyms
Evidence for Translation
Charge (pH 7)	-0.10
Kyte-Doolittle Hydrophobicity Value	-0.154
Molecular Weight (kDa)	35.8
Isoelectric Point (pI)	6.97

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3CRM	TRANSFERASE	04/07/08	Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	99.1
3CRR	TRANSFERASE	04/07/08	Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	99.1
3CRQ	TRANSFERASE	04/07/08	Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel	Pseudomonas aeruginosa	2.2	X-RAY DIFFRACTION	99.1

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 584 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004560 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_65320 Search term: miaA Search term: tRNA delta(2)-isopentenylpyrophosphate transferase

Human Homologs

References