Pseudomonas aeruginosa PAO1, PA4132 (mpaR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4132
Name
mpaR
Replicon chromosome
Genomic location 4621133 - 4622548 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentDeletion of mpaR caused increased biofilm formation, but reduced pyocyanin production (PMID:32748556)
CommentDeletion of mpaR leads to increased expression of the ant operon and the catechol production (PMID:32748556)

Cross-References

RefSeq NP_252821.1
GI 15599327
Affymetrix PA4132_at
Entrez 880050
GenBank AAG07519.1
INSDC AAG07519.1
NCBI Locus Tag PA4132
protein_id(GenBank) gb|AAG07519.1|AE004829_9|gnl|PseudoCAP|PA4132
TIGR NTL03PA04132
UniParc UPI00000C5C29
UniProtKB Acc Q9HWP9
UniProtKB ID Q9HWP9_PSEAE
UniRef100 UniRef100_Q9HWP9
UniRef50 UniRef50_Q4KCQ9
UniRef90 UniRef90_Q9HWP9

Product

Feature Type CDS
Coding Frame 1
Product Name
MpaR
Product Name Confidence: Class 1
Synonyms MvfR-mediated PQS and anthranilate regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.50
Kyte-Doolittle Hydrophobicity Value -0.099
Molecular Weight (kDa) 52.3
Isoelectric Point (pI) 8.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 617 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001567 (527 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4132
Search term: mpaR
Search term: MpaR

Human Homologs

References

A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T
DNA Res 1996 Dec 31;3(6):363-77
PubMed ID: 9097039
A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide.
Frangipani E, Pérez-Martínez I, Williams HD, Cherbuin G, Haas D
Environ Microbiol Rep 2014 Feb;6(1):28-34
PubMed ID: 24596260
Coordinated regulation of anthranilate metabolism and bacterial virulence by the GntR family regulator MpaR in Pseudomonas aeruginosa.
Wang T, Qi Y, Wang Z, Zhao J, Ji L, Li J, Cai Z, Yang L, Wu M, Liang H
Mol Microbiol 2020 Nov;114(5):857-869
PubMed ID: 32748556