Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0430
|
Name |
metF
|
Replicon | chromosome |
Genomic location | 481196 - 482068 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_249121.1 |
GI | 15595627 |
Affymetrix | PA0430_metF_at |
DNASU | PaCD00006182 |
Entrez | 877765 |
GenBank | AAG03819.1 |
INSDC | AAG03819.1 |
NCBI Locus Tag | PA0430 |
protein_id(GenBank) | gb|AAG03819.1|AE004480_3|gnl|PseudoCAP|PA0430 |
TIGR | NTL03PA00431 |
UniParc | UPI00000C504E |
UniProtKB Acc | Q9I687 |
UniProtKB ID | Q9I687_PSEAE |
UniRef100 | UniRef100_W0YKH2 |
UniRef50 | UniRef50_G2DES0 |
UniRef90 | UniRef90_A6UYP0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
5,10-methylenetetrahydrofolate reductase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -1.68 |
Kyte-Doolittle Hydrophobicity Value | -0.236 |
Molecular Weight (kDa) | 32.2 |
Isoelectric Point (pI) | 6.52 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 397 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000421 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0430
Search term: metF
Search term: 5,10-methylenetetrahydrofolate reductase
|
Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.
Saint-Girons I, Duchange N, Zakin MM, Park I, Margarita D, Ferrara P, Cohen GN
Nucleic Acids Res 1983 Oct 11;11(19):6723-32
PubMed ID: 6356036
|
Operator-constitutive mutations of the Escherichia coli metF gene.
Belfaiza J, Guillou Y, Margarita D, Perrin D, Saint Girons I
J Bacteriol 1987 Feb;169(2):670-4
PubMed ID: 3542965
|
Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli.
Sheppard CA, Trimmer EE, Matthews RG
J Bacteriol 1999 Feb;181(3):718-25
PubMed ID: 9922232
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|