Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1014
|
Name |
wapB
|
Replicon | chromosome |
Genomic location | 1097296 - 1098288 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 7 transposon mutants in orthologs |
Comment | Responsible for transfer of the terminal glucose residue to the outer core oligosaccharide in P. aeruginosa PAO1 LPS. In a knockout-mutant, the chemical structure of the LPS extracted from it lacked the terminal glucose residue as compared to the core oligosaccharide structure of the wild type strain PAO1. |
RefSeq | NP_249705.1 |
GI | 15596211 |
Affymetrix | PA1014_at |
DNASU | PaCD00006467 |
Entrez | 883119 |
GenBank | AAG04403.1 |
NCBI Locus Tag | PA1014 |
protein_id(GenBank) | gb|AAG04403.1|AE004534_1|gnl|PseudoCAP|PA1014 |
TIGR | NTL03PA01015 |
UniProtKB Acc | Q9I4V9 |
UniProtKB ID | Q9I4V9_PSEAE |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
1,2-glucosyltransferase WapB
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 4.50 |
Kyte-Doolittle Hydrophobicity Value | -0.272 |
Molecular Weight (kDa) | 37.3 |
Isoelectric Point (pI) | 8.33 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 551 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000682 (2121 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1014
Search term: wapB
Search term: 1,2-glucosyltransferase WapB
|
Evidence that WapB is a 1,2-glucosyltransferase of Pseudomonas aeruginosa involved in Lipopolysaccharide outer core biosynthesis.
Kocíncová D, Hao Y, Vinogradov E, Lam JS
J Bacteriol 2011 Jun;193(11):2708-16
PubMed ID: 21441506
|