Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1032
|
Name |
quiP
Synonym: pac |
Replicon | chromosome |
Genomic location | 1119674 - 1122217 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Encodes an enzyme with a homoserine lactone-releasing, acyl-homoserine lactone(AHL)acylase activity specific towards long-chain AHLs |
Comment | quiP encodes a protein with acyl-HSL acylase activity for long but not short acyl-HSLs. |
RefSeq | NP_249723.1 |
GI | 15596229 |
Affymetrix | PA1032_at |
Entrez | 880272 |
GenBank | AAG04421.1 |
INSDC | AAG04421.1 |
NCBI Locus Tag | PA1032 |
protein_id(GenBank) | gb|AAG04421.1|AE004535_8|gnl|PseudoCAP|PA1032 |
TIGR | NTL03PA01033 |
UniParc | UPI00000C5213 |
UniProtKB Acc | Q9I4U2 |
UniProtKB ID | QUIP_PSEAE |
UniRef100 | UniRef100_Q9I4U2 |
UniRef50 | UniRef50_Q9I4U2 |
UniRef90 | UniRef90_Q9I4U2 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
QuiP
|
Synonyms |
acyl-homoserine lactone acylase, QuiP QuiP (changed from probable penicillin amidase) |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 3.31 |
Kyte-Doolittle Hydrophobicity Value | -0.410 |
Molecular Weight (kDa) | 94.1 |
Isoelectric Point (pI) | 7.75 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)
|
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000994 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1032
Search term: quiP
Search term: QuiP
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosa PAO1.
Huang JJ, Petersen A, Whiteley M, Leadbetter JR
Appl Environ Microbiol 2006 Feb;72(2):1190-7
PubMed ID: 16461666
|
Penicillin acylase from E. coli: unique gene-protein relation.
Schumacher G, Sizmann D, Haug H, Buckel P, Böck A
Nucleic Acids Res 1986 Jul 25;14(14):5713-27
PubMed ID: 3016663
|
Cloning and characterization of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.
Matsuda A, Matsuyama K, Yamamoto K, Ichikawa S, Komatsu K
J Bacteriol 1987 Dec;169(12):5815-20
PubMed ID: 2824449
|
Nucleotide sequences of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.
Matsuda A, Toma K, Komatsu K
J Bacteriol 1987 Dec;169(12):5821-6
PubMed ID: 3680178
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|