Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4251
|
Name |
rplE
|
Replicon | chromosome |
Genomic location | 4761381 - 4761920 (- strand) |
RefSeq | NP_252941.1 |
GI | 15599447 |
Affymetrix | PA4251_rplE_at |
DNASU | PaCD00007846 |
Entrez | 881779 |
GenBank | AAG07639.1 |
INSDC | AAG07639.1 |
NCBI Locus Tag | PA4251 |
protein_id(GenBank) | gb|AAG07639.1|AE004841_17|gnl|PseudoCAP|PA4251 |
TIGR | NTL03PA04252 |
UniParc | UPI00000C5C85 |
UniProtKB Acc | Q9HWE7 |
UniProtKB ID | RL5_PSEAE |
UniRef100 | UniRef100_B7V656 |
UniRef50 | UniRef50_P08895 |
UniRef90 | UniRef90_Q4K545 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
50S ribosomal protein L5
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using LC-MS/MS (20.39 kDa, PMID:21751344)
|
Charge (pH 7) | 8.00 |
Kyte-Doolittle Hydrophobicity Value | -0.313 |
Molecular Weight (kDa) | 20.4 |
Isoelectric Point (pI) | 10.30 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 99.4 |
6SPB | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 | Pseudomonas aeruginosa | 2.82 | ELECTRON MICROSCOPY | 98.3 |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
6SPD | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.28 | ELECTRON MICROSCOPY | 99.4 |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 99.4 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001459 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4251
Search term: rplE
Search term: 50S ribosomal protein L5
|
The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes.
Chen R, Ehrke G
FEBS Lett 1976 Oct 15;69(1):240-5
PubMed ID: 791672
|
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M
Nucleic Acids Res 1983 May 11;11(9):2599-616
PubMed ID: 6222285
|
Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.
Huber PW, Wool IG
Proc Natl Acad Sci U S A 1984 Jan;81(2):322-6
PubMed ID: 6364140
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|