Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4262
|
Name |
rplD
|
Replicon | chromosome |
Genomic location | 4766009 - 4766611 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
RefSeq | NP_252952.1 |
GI | 15599458 |
Affymetrix | PA4262_rplD_at |
DNASU | PaCD00007850 |
Entrez | 881754 |
GenBank | AAG07650.1 |
INSDC | AAG07650.1 |
NCBI Locus Tag | PA4262 |
protein_id(GenBank) | gb|AAG07650.1|AE004841_28|gnl|PseudoCAP|PA4262 |
TIGR | NTL03PA04263 |
UniParc | UPI00000C5C8C |
UniProtKB Acc | Q9HWD6 |
UniProtKB ID | RL4_PSEAE |
UniRef100 | UniRef100_A6UZI9 |
UniRef50 | UniRef50_A6UZI9 |
UniRef90 | UniRef90_A6UZI9 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
50S ribosomal protein L4
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using LC-MS/MS (21.64 kDa, PMID:21751344)
|
Charge (pH 7) | 10.71 |
Kyte-Doolittle Hydrophobicity Value | -0.209 |
Molecular Weight (kDa) | 21.6 |
Isoelectric Point (pI) | 10.56 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
6SPB | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 | Pseudomonas aeruginosa | 2.82 | ELECTRON MICROSCOPY | 100.0 |
6SPD | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.28 | ELECTRON MICROSCOPY | 100.0 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 100.0 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 100.0 |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001448 (528 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4262
Search term: rplD
Search term: 50S ribosomal protein L4
|
Ensembl
110, assembly
GRCh38.p14
|
mitochondrial ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:14276]
E-value:
1.9e-10
Percent Identity:
28.5
|
Ensembl
110, assembly
GRCh38.p14
|
mitochondrial ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:14276]
E-value:
1.9e-10
Percent Identity:
28.5
|
Ensembl
110, assembly
GRCh38.p14
|
mitochondrial ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:14276]
E-value:
1.9e-10
Percent Identity:
28.5
|
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
|
Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation.
Freedman LP, Zengel JM, Archer RH, Lindahl L
Proc Natl Acad Sci U S A 1987 Sep;84(18):6516-20
PubMed ID: 2442760
|
Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions.
Li X, Lindahl L, Zengel JM
RNA 1996 Jan;2(1):24-37
PubMed ID: 8846294
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|