Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4700
|
Name |
mrcB
Synonym: pbpF Synonym: ponB |
Replicon | chromosome |
Genomic location | 5277968 - 5280292 (- strand) |
Transposon Mutants | 7 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
RefSeq | NP_253388.1 |
GI | 15599894 |
Affymetrix | PA4700_mrcB_at |
Entrez | 881511 |
GenBank | AAG08086.1 |
INSDC | AAG08086.1 |
NCBI Locus Tag | PA4700 |
protein_id(GenBank) | gb|AAG08086.1|AE004884_4|gnl|PseudoCAP|PA4700 |
TIGR | NTL03PA04701 |
UniParc | UPI00000D424A |
UniProtKB Acc | G3XD31 |
UniProtKB ID | G3XD31_PSEAE |
UniRef100 | UniRef100_G3XD31 |
UniRef50 | UniRef50_Q4K603 |
UniRef90 | UniRef90_A6VCF4 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
penicillin-binding protein 1B
|
Synonyms |
peptidoglycan synthetase |
Evidence for Translation |
Detected using LC-MS/MS (85.49 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 11.89 |
Kyte-Doolittle Hydrophobicity Value | -0.282 |
Molecular Weight (kDa) | 85.5 |
Isoelectric Point (pI) | 9.57 |
Individual Mappings | |
Additional evidence for subcellular localization |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Ceftolozane | DB09050 | DrugBank | 5.1.4 | Q9X6W0 | Penicillin-binding protein 1B | 100.0 | 774 | 0.0e+00 |
Ceftazidime | DB00438 | DrugBank | 5.1.4 | Q9X6W0 | Penicillin-binding protein 1B | 100.0 | 774 | 0.0e+00 |
Cefoperazone | DB01329 | DrugBank | 5.1.4 | Q9X6W0 | Penicillin-binding protein 1B | 100.0 | 774 | 0.0e+00 |
Cefpiramide | DB00430 | DrugBank | 5.1.4 | Q9X6W0 | Penicillin-binding protein 1B | 100.0 | 774 | 0.0e+00 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 553 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004349 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4700
Search term: mrcB
Search term: penicillin-binding protein 1B
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
In vitro peptidoglycan polymerization catalysed by penicillin binding protein 1b of Escherichia coli K-12.
Suzuki H, van Heijenoort Y, Tamura T, Mizoguchi J, Hirota Y, van Heijenoort J
FEBS Lett 1980 Feb 11;110(2):245-9
PubMed ID: 6989636
|
Molecular divergence of a major peptidoglycan synthetase with transglycosylase-transpeptidase activities in Escherichia coli --- penicillin-binding protein 1Bs.
Nakagawa J, Matsuhashi M
Biochem Biophys Res Commun 1982 Apr 29;105(4):1546-53
PubMed ID: 7049165
|
Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase.
Nakagawa J, Tamaki S, Tomioka S, Matsuhashi M
J Biol Chem 1984 Nov 25;259(22):13937-46
PubMed ID: 6389538
|
Purification and sequencing of the active site tryptic peptide from penicillin-binding protein 1b of Escherichia coli.
Nicholas RA, Suzuki H, Hirota Y, Strominger JL
Biochemistry 1985 Jul 2;24(14):3448-53
PubMed ID: 3899167
|
Topographical and functional investigation of Escherichia coli penicillin-binding protein 1b by alanine stretch scanning mutagenesis.
Lefèvre F, Rémy MH, Masson JM
J Bacteriol 1997 Aug;179(15):4761-7
PubMed ID: 9244263
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|