Pseudomonas aeruginosa PAO1, PA0003 (recF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0003
Name
recF
Replicon chromosome
Genomic location 3169 - 4278 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_064723.1
GI 15595201
Affymetrix PA0003_recF_at
DNASU PaCD00006005
Entrez 879229
GenBank AAG03393.1
INSDC AAG03393.1
NCBI Locus Tag PA0003
protein_id(GenBank) gb|AAG03393.1|AE004440_3|gnl|PseudoCAP|PA0003
TIGR NTL03PA00004
UniParc UPI0000133565
UniProtKB Acc Q9I7C3
UniProtKB ID RECF_PSEAE
UniRef100 UniRef100_Q02V78
UniRef50 UniRef50_P13456
UniRef90 UniRef90_Q02V78

Product

Feature Type CDS
Coding Frame 1
Product Name
RecF protein
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.23
Kyte-Doolittle Hydrophobicity Value -0.249
Molecular Weight (kDa) 41.5
Isoelectric Point (pI) 8.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 464 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000003 (571 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0003
Search term: recF
Search term: RecF protein

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 48686
Genomic Position 3271
Position in ORF 103(1110)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 49647
Genomic Position 3271
Position in ORF 103(1110)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_00030
Mutant ID PA14NR: 25777
Genomic Position 3676
Position in ORF 508(1110)
Reference 16477005

References

Structure of the dnaA region of Pseudomonas putida: conservation among three bacteria, Bacillus subtilis, Escherichia coli and P. putida.
Fujita MQ, Yoshikawa H, Ogasawara N.
Mol Gen Genet. 1989 Feb;215(3):381-7.
PubMed ID: 2540413
Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop.
Sandler SJ, Chackerian B, Li JT, Clark AJ.
Nucleic Acids Res. 1992 Feb 25;20(4):839-45.
PubMed ID: 1542576