Pseudomonas aeruginosa PAO1, PA0010 (tag)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0010
Name
tag
Replicon chromosome
Genomic location 13540 - 14091 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248700.1
GI 15595208
Affymetrix PA0010_tag_at
DNASU PaCD00006007
Entrez 878076
GenBank AAG03400.1
INSDC AAG03400.1
NCBI Locus Tag PA0010
protein_id(GenBank) gb|AAG03400.1|AE004441_1|gnl|PseudoCAP|PA0010
TIGR NTL03PA00011
UniParc UPI00000C4EE5
UniProtKB Acc Q9I7B6
UniProtKB ID Q9I7B6_PSEAE
UniRef100 UniRef100_W0YLR1
UniRef50 UniRef50_A0A090ITZ8
UniRef90 UniRef90_A6UX71

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-3-methyladenine glycosidase I
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -7.40
Kyte-Doolittle Hydrophobicity Value -0.225
Molecular Weight (kDa) 20.8
Isoelectric Point (pI) 4.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 355 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000010 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0010
Search term: tag

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 54142
Genomic Position 13739
Position in ORF 200(552)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 50564
Genomic Position 13671
Position in ORF 132(552)
Reference 14617778

Transposon Insertions in Orthologs

References

Nucleotide sequence of the tag gene from Escherichia coli.
Steinum AL, Seeberg E
Nucleic Acids Res. 1986 May;14(9):3763-72
PubMed ID: 3520491
Purification and structure of 3-methyladenine-DNA glycosylase I of Escherichia coli.
Sakumi K, Nakabeppu Y, Yamamoto Y, Kawabata S, Iwanaga S, Sekiguchi M
J. Biol. Chem. 1986 Nov;261(33):15761-6
PubMed ID: 3536912
Different efficiencies of the Tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3-methyladenine from single-stranded DNA.
Bjelland S, Seeberg E
FEBS Lett. 1996 Nov;397(1):127-9
PubMed ID: 8941728