Pseudomonas aeruginosa PAO1, PA0010 (tag)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0010
Name
tag
Replicon chromosome
Genomic location 13540 - 14091 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248700.1
GI 15595208
Affymetrix PA0010_tag_at
DNASU PaCD00006007
Entrez 878076
GenBank AAG03400.1
INSDC AAG03400.1
NCBI Locus Tag PA0010
protein_id(GenBank) gb|AAG03400.1|AE004441_1|gnl|PseudoCAP|PA0010
TIGR NTL03PA00011
UniParc UPI00000C4EE5
UniProtKB Acc Q9I7B6
UniProtKB ID Q9I7B6_PSEAE
UniRef100 UniRef100_W0YLR1
UniRef50 UniRef50_A0A090ITZ8
UniRef90 UniRef90_A6UX71

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-3-methyladenine glycosidase I
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -7.40
Kyte-Doolittle Hydrophobicity Value -0.225
Molecular Weight (kDa) 20.8
Isoelectric Point (pI) 4.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 355 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000010 (572 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0010
Search term: tag

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 50564
Genomic Position 13671
Position in ORF 132(552)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 54142
Genomic Position 13739
Position in ORF 200(552)
Reference 14617778

Transposon Insertions in Orthologs

References

Nucleotide sequence of the tag gene from Escherichia coli.
Steinum AL, Seeberg E.
Nucleic Acids Res. 1986 May 12;14(9):3763-72.
PubMed ID: 3520491
Purification and structure of 3-methyladenine-DNA glycosylase I of Escherichia coli.
Sakumi K, Nakabeppu Y, Yamamoto Y, Kawabata S, Iwanaga S, Sekiguchi M.
J Biol Chem. 1986 Nov 25;261(33):15761-6.
PubMed ID: 3536912
Different efficiencies of the Tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3-methyladenine from single-stranded DNA.
Bjelland S, Seeberg E.
FEBS Lett. 1996 Nov 11;397(1):127-9.
PubMed ID: 8941728