Pseudomonas aeruginosa PAO1, PA0091 (vgrG1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0091
Name
vgrG1
Synonym: vgrG1a
Replicon chromosome
Genomic location 110976 - 112907 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_248781.1
GI 15595289
Affymetrix PA0091_at
Entrez 879511
GenBank AAG03481.1
INSDC AAG03481.1
NCBI Locus Tag PA0091
protein_id(GenBank) gb|AAG03481.1|AE004447_14|gnl|PseudoCAP|PA0091
TIGR NTL03PA00092
UniParc UPI00000C4F2C
UniProtKB Acc Q9I741
UniProtKB ID Q9I741_PSEAE
UniRef100 UniRef100_Q9I741
UniRef50 UniRef50_Q9I741
UniRef90 UniRef90_Q9I741

Product

Feature Type CDS
Coding Frame 1
Product Name
VgrG1
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.10
Kyte-Doolittle Hydrophobicity Value -0.542
Molecular Weight (kDa) 72.0
Isoelectric Point (pI) 6.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4MTK TOXIN 09/19/13 Crystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion System PSEUDOMONAS AERUGINOSA 3.322 X-RAY DIFFRACTION 100.0
4UHV STRUCTURAL PROTEIN 03/25/15 The structure of VgrG1, the needle tip of the bacterial Type VI Secretion System PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 156 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000090 (3427 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0091
Search term: vgrG1
Search term: VgrG1

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_32_E11
Genomic Position 112732
Position in ORF 1757(1932)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 43832
Genomic Position 112112
Position in ORF 1137(1932)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 34399
Genomic Position 111084
Position in ORF 109(1932)
Reference 14617778

Transposon Insertions in Orthologs

References

Rhs elements comprise three subfamilies which diverged prior to acquisition by Escherichia coli.
Wang YD, Zhao S, Hill CW
J. Bacteriol. 1998 Aug;180(16):4102-10
PubMed ID: 9696756
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun;312(5779):1526-30
PubMed ID: 16763151
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jové D, Filloux A
J. Biol. Chem. 2011 Apr;286(14):12317-27
PubMed ID: 21325275
An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells.
Whitney JC, Quentin D, Sawai S, LeRoux M, Harding BN, Ledvina HE, Tran BQ, Robinson H, Goo YA, Goodlett DR, Raunser S, Mougous JD
Cell 2015 Oct;163(3):607-19
PubMed ID: 26456113