Pseudomonas aeruginosa PAO1, PA0143 (nuh)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0143
Name
nuh
Replicon chromosome
Genomic location 163363 - 164415 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248833.2
GI 119167454
Affymetrix PA0143_at
DNASU PaCD00006062
Entrez 879451
GenBank AAG03533.2
INSDC AAG03533.2
NCBI Locus Tag PA0143
protein_id(GenBank) gb|AAG03533.2|AE004452_5|gnl|PseudoCAP|PA0143
TIGR NTL03PA00144
UniParc UPI00000077AE
UniProtKB Acc Q9I6Y9
UniProtKB ID Q9I6Y9_PSEAE
UniRef100 UniRef100_Q9I6Y9
UniRef50 UniRef50_Q9I6Y9
UniRef90 UniRef90_Q9I6Y9

Product

Feature Type CDS
Coding Frame 1
Product Name
purine nucleosidase Nuh
Product Name Confidence: Class 1
CommentSpecific function outlined in reference. Nonspecific ribonucleoside hydrolase annotation ID (with class I confidence) implies that there are more reactions catalyzed by this enzyme.
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.38
Kyte-Doolittle Hydrophobicity Value 0.029
Molecular Weight (kDa) 37.5
Isoelectric Point (pI) 7.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 241 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000141 (492 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0143
Search term: nuh

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 33313
Genomic Position 164362
Position in ORF 1000(1053)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3283
Genomic Position 164329
Position in ORF 967(1053)
Reference 14617778

Transposon Insertions in Orthologs

References

Impact of quorum sensing on fitness of Pseudomonas aeruginosa.
Heurlier K, Dénervaud V, Haas D
Int. J. Med. Microbiol. 2006 Apr;296(2-3):93-102
PubMed ID: 16503417
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994