Pseudomonas aeruginosa PAO1, PA0211 (mdcD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0211
Name
mdcD
Replicon chromosome
Genomic location 240071 - 240934 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248902.1
GI 15595408
Affymetrix PA0211_mdcD_at
Entrez 879489
GenBank AAG03600.1
INSDC AAG03600.1
NCBI Locus Tag PA0211
protein_id(GenBank) gb|AAG03600.1|AE004459_4|gnl|PseudoCAP|PA0211
TIGR NTL03PA00212
UniParc UPI00000C4F9A
UniProtKB Acc Q9I6S7
UniProtKB ID Q9I6S7_PSEAE
UniRef100 UniRef100_Q9I6S7
UniRef50 UniRef50_A0A085GVQ3
UniRef90 UniRef90_A6UY06

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate decarboxylase beta subunit
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.08
Kyte-Doolittle Hydrophobicity Value 0.041
Molecular Weight (kDa) 30.3
Isoelectric Point (pI) 4.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 556 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000210 (505 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0211
Search term: mdcD

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_32_D2
Genomic Position 240143
Position in ORF 73(864)
Reference 15805499
Type ISlacZ/hah insertion site
Mutant ID UWGC: 3154
Genomic Position 240329
Position in ORF 259(864)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 46646
Genomic Position 240622
Position in ORF 552(864)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02580
Mutant ID PA14NR: 29777
Genomic Position 230734
Position in ORF 513(864)
Reference 16477005

References

Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli.
Hoenke S, Schmid M, Dimroth P.
Eur J Biochem. 1997 Jun 1;246(2):530-8.
PubMed ID: 9208947