Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0297
|
Name |
spuA
Synonym: ycjL |
Replicon | chromosome |
Genomic location | 334734 - 335486 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_248988.1 |
GI | 15595494 |
Affymetrix | PA0297_at |
DNASU | PaCD00006130 |
Entrez | 880604 |
GenBank | AAG03686.1 |
INSDC | AAG03686.1 |
NCBI Locus Tag | PA0297 |
protein_id(GenBank) | gb|AAG03686.1|AE004467_8|gnl|PseudoCAP|PA0297 |
TIGR | NTL03PA00298 |
UniParc | UPI00000C4FEC |
UniProtKB Acc | Q9I6J4 |
UniProtKB ID | Q9I6J4_PSEAE |
UniRef100 | UniRef100_W0YL61 |
UniRef50 | UniRef50_Q48IV7 |
UniRef90 | UniRef90_W0YL61 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
probable glutamine amidotransferase
|
Synonyms |
pauD1 |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -3.45 |
Kyte-Doolittle Hydrophobicity Value | -0.001 |
Molecular Weight (kDa) | 26.5 |
Isoelectric Point (pI) | 6.43 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7D50 | HYDROLASE | 09/24/20 | SpuA mutant - H221N with glutamyl-thioester | Pseudomonas aeruginosa PAO1 | 1.77 | X-RAY DIFFRACTION | 99.6 |
7D4R | HYDROLASE | 09/24/20 | SpuA native structure | Pseudomonas aeruginosa PAO1 | 1.6 | X-RAY DIFFRACTION | 100.0 |
7D53 | HYDROLASE | 09/24/20 | SpuA mutant - H221N with Glu | Pseudomonas aeruginosa PAO1 | 1.6 | X-RAY DIFFRACTION | 99.6 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 316 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000294 (491 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0297
Search term: spuA
Search term: probable glutamine amidotransferase
|
Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.
Yao X, He W, Lu CD
J. Bacteriol. 2011 Aug;193(15):3923-30
PubMed ID: 21622750
|