Pseudomonas aeruginosa PAO1, PA0546 (metK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0546
Name
metK
Replicon chromosome
Genomic location 603706 - 604896 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249237.1
GI 15595743
Affymetrix PA0546_metK_at
DNASU PaCD00006236
Entrez 878379
GenBank AAG03935.1
INSDC AAG03935.1
NCBI Locus Tag PA0546
protein_id(GenBank) gb|AAG03935.1|AE004491_2|gnl|PseudoCAP|PA0546
TIGR NTL03PA00547
UniParc UPI000012EFD0
UniProtKB Acc Q9I5Z0
UniProtKB ID METK_PSEAE
UniRef100 UniRef100_A6UZ09
UniRef50 UniRef50_P13444
UniRef90 UniRef90_Q4K4I7

Product

Feature Type CDS
Coding Frame 1
Product Name
methionine adenosyltransferase
Product Name Confidence: Class 2
Synonyms AdoMet synthetase
S-adenosylmethionine synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.77
Kyte-Doolittle Hydrophobicity Value -0.103
Molecular Weight (kDa) 42.7
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000530 (559 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0546
Search term: metK

Human Homologs

Ensembl 86, assembly GRCh38.p7
methionine adenosyltransferase 2A [Source:HGNC Symbol;Acc:HGNC:6904]
E-value: 2.5e-113
Percent Identity: 56.8

References

The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli.
Markham GD, DeParasis J, Gatmaitan J
J. Biol. Chem. 1984 Dec;259(23):14505-7
PubMed ID: 6094561
Crystal structure of S-adenosylmethionine synthetase.
Takusagawa F, Kamitori S, Misaki S, Markham GD
J. Biol. Chem. 1996 Jan;271(1):136-47
PubMed ID: 8550549