Pseudomonas aeruginosa PAO1, PA3439 (folX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3439
Name
folX
Replicon chromosome
Genomic location 3846337 - 3846708 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252129.1
GI 15598635
Affymetrix PA3439_folX_at
DNASU PaCD00007513
Entrez 877686
GenBank AAG06827.1
INSDC AAG06827.1
NCBI Locus Tag PA3439
protein_id(GenBank) gb|AAG06827.1|AE004764_13|gnl|PseudoCAP|PA3439
TIGR NTL03PA03439
UniParc UPI00000C5A10
UniProtKB Acc Q9HYG7
UniProtKB ID Q9HYG7_PSEAE
UniRef100 UniRef100_W0YVQ0
UniRef50 UniRef50_P0AC21
UniRef90 UniRef90_A6V1Y8

Product

Feature Type CDS
Coding Frame 1
Product Name
d-erythro-7,8-dihydroneopterin triphosphate epimerase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.04
Kyte-Doolittle Hydrophobicity Value -0.133
Molecular Weight (kDa) 14.2
Isoelectric Point (pI) 6.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4AEY ISOMERASE 01/13/12 Crystal structure of FolX from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 3 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 139 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002197 (474 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3439
Search term: folX

Human Homologs

References

Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.
Haussmann C, Rohdich F, Lottspeich F, Eberhardt S, Scheuring J, Mackamul S, Bacher A
J Bacteriol 1997 Feb;179(3):949-51
PubMed ID: 9006053
Purification, cloning, and functional expression of dihydroneopterin triphosphate 2'-epimerase from Escherichia coli.
Ahn C, Byun J, Yim J
J Biol Chem 1997 Jun 13;272(24):15323-8
PubMed ID: 9182560
FolX and FolM are essential for tetrahydromonapterin synthesis in Escherichia coli and Pseudomonas aeruginosa.
Pribat A, Blaby IK, Lara-Núñez A, Gregory JF 3rd, de Crécy-Lagard V, Hanson AD
J Bacteriol 2010 Jan;192(2):475-82
PubMed ID: 19897652