Pseudomonas aeruginosa PAO1, PA3648 (opr86)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3648
Name
opr86
Replicon chromosome
Genomic location 4085062 - 4087455 (- strand)
Commentessential for viability and a role in outer membrane protein assembly

Cross-References

RefSeq NP_252338.1
GI 15598844
Affymetrix PA3648_at
Entrez 880506
GenBank AAG07036.1
INSDC AAG07036.1
NCBI Locus Tag PA3648
protein_id(GenBank) gb|AAG07036.1|AE004784_9|gnl|PseudoCAP|PA3648
TIGR NTL03PA03648
UniParc UPI00000C5AA3
UniProtKB Acc Q9HXY4
UniProtKB ID Q9HXY4_PSEAE
UniRef100 UniRef100_Q9HXY4
UniRef50 UniRef50_Q9HXY4
UniRef90 UniRef90_Q9HXY4

Product

Feature Type CDS
Coding Frame 1
Product Name
outer membrane protein Opr86
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (88.29 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -18.57
Kyte-Doolittle Hydrophobicity Value -0.368
Molecular Weight (kDa) 88.3
Isoelectric Point (pI) 4.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 411 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002026 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3648
Search term: opr86

Human Homologs

References

Opr86 is essential for viability and is a potential candidate for a protective antigen against biofilm formation by Pseudomonas aeruginosa.
Tashiro Y, Nomura N, Nakao R, Senpuku H, Kariyama R, Kumon H, Kosono S, Watanabe H, Nakajima T, Uchiyama H
J Bacteriol 2008 Jun;190(11):3969-78
PubMed ID: 18390657
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344