Pseudomonas aeruginosa PAO1, PA4268 (rpsL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4268
Name
rpsL
Synonym: str
Replicon chromosome
Genomic location 4771756 - 4772127 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252958.1
GI 15599464
Affymetrix PA4268_rpsL_at
DNASU PaCD00007852
Entrez 881709
GenBank AAG07656.1
INSDC AAG07656.1
NCBI Locus Tag PA4268
protein_id(GenBank) gb|AAG07656.1|AE004842_5|gnl|PseudoCAP|PA4268
TIGR NTL03PA04269
UniParc UPI0000134C69
UniProtKB Acc Q9HWD0
UniProtKB ID RS12_PSEAE
UniRef100 UniRef100_A6UZI3
UniRef50 UniRef50_P61941
UniRef90 UniRef90_A6W397

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S12
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 20.61
Kyte-Doolittle Hydrophobicity Value -0.818
Molecular Weight (kDa) 13.8
Isoelectric Point (pI) 11.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 660 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001443 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4268
Search term: rpsL

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5

References

The nucleotide sequence of rpsL and its flanking regions in Salmonella typhimurium.
Hughes D, Buckingham RH
Gene 1991 Jul 31;104(1):123-4
PubMed ID: 1916272
Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis.
Timms AR, Steingrimsdottir H, Lehmann AR, Bridges BA
Mol Gen Genet 1992 Mar;232(1):89-96
PubMed ID: 1552908
Modelling in Escherichia coli of mutations in mitoribosomal protein S12: novel mutant phenotypes of rpsL.
Toivonen JM, Boocock MR, Jacobs HT
Mol Microbiol 1999 Mar;31(6):1735-46
PubMed ID: 10209746
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142