Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4671
|
Name |
Synonym: rplY |
Replicon | chromosome |
Genomic location | 5239466 - 5240080 (+ strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_253360.1 |
GI | 15599866 |
Affymetrix | PA4671_at |
DNASU | PaCD00008028 |
Entrez | 881395 |
GenBank | AAG08058.1 |
INSDC | AAG08058.1 |
NCBI Locus Tag | PA4671 |
protein_id(GenBank) | gb|AAG08058.1|AE004881_1|gnl|PseudoCAP|PA4671 |
TIGR | NTL03PA04672 |
UniParc | UPI00000C5DB8 |
UniProtKB Acc | Q9HVC4 |
UniProtKB ID | RL25_PSEAE |
UniRef100 | UniRef100_B7V0L7 |
UniRef50 | UniRef50_Q02G03 |
UniRef90 | UniRef90_Q02G03 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
probable ribosomal protein L25
|
Synonyms | |
Evidence for Translation |
Detected by iTRAQ (PMID:21863142).
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected using LC-MS/MS (21.96 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -4.62 |
Kyte-Doolittle Hydrophobicity Value | 0.030 |
Molecular Weight (kDa) | 22.0 |
Isoelectric Point (pI) | 6.17 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 98.9 |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
6SPD | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.28 | ELECTRON MICROSCOPY | 98.9 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 98.9 |
6SPB | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 | Pseudomonas aeruginosa | 2.82 | ELECTRON MICROSCOPY | 98.9 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 517 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004321 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4671
Search term: probable ribosomal protein L25
|
The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases.
Stoldt M, Wöhnert J, Görlach M, Brown LR
EMBO J 1998 Nov 2;17(21):6377-84
PubMed ID: 9799245
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|