Pseudomonas aeruginosa PAO1, PA0519 (nirS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0519
Name
nirS
Replicon chromosome
Genomic location 578394 - 580100 (- strand)
Transposon Mutants 13 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249210.1
GI 15595716
Affymetrix PA0519_nirS_at
Entrez 882217
GenBank AAG03908.1
INSDC CAA35702.1
NCBI Locus Tag PA0519
protein_id(GenBank) gb|AAG03908.1|AE004488_12|gnl|PseudoCAP|PA0519
TIGR NTL03PA00520
UniParc UPI0000055F2D
UniProtKB Acc P24474
UniProtKB ID NIRS_PSEAE
UniRef100 UniRef100_P24474
UniRef50 UniRef50_P24474
UniRef90 UniRef90_P24474

Product

Feature Type CDS
Coding Frame 1
Product Name
nitrite reductase precursor
Product Name Confidence: Class 1
Synonyms cytochrome oxidase
cytochrome cd1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 5.04
Kyte-Doolittle Hydrophobicity Value -0.391
Molecular Weight (kDa) 62.7
Isoelectric Point (pI) 8.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1NIR NITRITE REDUCTASE 06/17/97 OXYDIZED NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.15 X-RAY DIFFRACTION 100.0
1GJQ OXIDOREDUCTASE 08/01/01 Pseudomonas aeruginosa cd1 nitrite reductase reduced cyanide complex PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 100.0
1N90 OXIDOREDUCTASE 09/04/98 FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
1BL9 OXIDOREDUCTASE 07/20/98 CONFORMATIONAL CHANGES OCCURRING UPON REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
1N50 OXIDOREDUCTASE 09/04/98 FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
1N15 OXIDOREDUCTASE 09/04/98 FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
1HZV OXIDOREDUCTASE 01/26/01 DOMAIN SWING UPON HIS TO ALA MUTATION IN NITRITE REDUCTASE OF PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.83 X-RAY DIFFRACTION 99.8
1NNO OXIDOREDUCTASE 07/20/98 CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.65 X-RAY DIFFRACTION 100.0
1HZU OXIDOREDUCTASE 01/26/01 DOMAIN SWING UPON HIS TO ALA MUTATION IN NITRITE REDUCTASE OF PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 99.8
5GUW MEMBRANE PROTEIN 08/31/16 Complex of Cytochrome cd1 Nitrite Reductase and Nitric Oxide Reductase in Denitrification of Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 3.2 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Heme D DB03469 DrugBank 5.1.1 P24474 Nitrite reductase 100.0 568 0.0e+00
Heme C DB03317 DrugBank 5.1.1 P24474 Nitrite reductase 100.0 568 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000503 (364 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0519
Search term: nirS

References

Nitrite reductase from Pseudomonas aeruginosa: sequence of the gene and the protein.
Silvestrini MC, Galeotti CL, Gervais M, Schininà E, Barra D, Bossa F, Brunori M
FEBS Lett. 1989 Aug;254(1-2):33-8
PubMed ID: 2506077
The structural gene for cytochrome c551 from Pseudomonas aeruginosa. The nucleotide sequence shows a location downstream of the nitrite reductase gene.
Nordling M, Young S, Karlsson BG, Lundberg LG
FEBS Lett. 1990 Jan;259(2):230-2
PubMed ID: 2152881
N-terminal arm exchange is observed in the 2.15 A crystal structure of oxidized nitrite reductase from Pseudomonas aeruginosa.
Nurizzo D, Silvestrini MC, Mathieu M, Cutruzzolà F, Bourgeois D, Fülöp V, Hajdu J, Brunori M, Tegoni M, Cambillau C
Structure 1997 Sep;5(9):1157-71
PubMed ID: 9331415
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
NO production by Pseudomonas aeruginosa cd1 nitrite reductase.
Cutruzzolà F, Rinaldo S, Centola F, Brunori M
IUBMB Life ;55(10-11):617-21
PubMed ID: 14711008