Pseudomonas aeruginosa PAO1, PA1254 (lhpC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 1362371 - 1363288 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249945.1
GI 15596451
Affymetrix PA1254_at
DNASU PaCD00006579
Entrez 881320
GenBank AAG04643.1
INSDC AAG04643.1
NCBI Locus Tag PA1254
protein_id(GenBank) gb|AAG04643.1|AE004555_3|gnl|PseudoCAP|PA1254
UniParc UPI00000C52D0
UniProtKB Acc Q9I490
UniRef100 UniRef100_A0A0C7D604
UniRef50 UniRef50_U1LTR1
UniRef90 UniRef90_A0A022NV25


Feature Type CDS
Coding Frame 1
Product Name
delta1-pyrroline-4-hydroxy-2-carboxylate deaminase, LphC
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -5.73
Kyte-Doolittle Hydrophobicity Value 0.107
Molecular Weight (kDa) 32.9
Isoelectric Point (pI) 5.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 632 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000222 (1196 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1254
Search term: lhpC

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8867
Genomic Position 1362606
Position in ORF 236(918)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 1840
Genomic Position 1363183
Position in ORF 813(918)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_48000
Mutant ID PA14NR: 44794
Genomic Position 4271839
Position in ORF 533(918)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_48000
Mutant ID PA14NR: 48613
Genomic Position 4271625
Position in ORF 319(918)
Reference 16477005


Molecular characterization of LhpR in control of hydroxyproline catabolism and transport in Pseudomonas aeruginosa PAO1.
Li G(1), Lu CD(2).
Microbiology. 2016 Jul;162(7):1232-42.
PubMed ID: 27145750
Alpha-ketoglutaric semialdehyde dehydrogenase of Pseudomonas. Properties of the purified enzyme induced by hydroxyproline and of the glucarate-induced and constitutive enzymes.
Adams E, Rosso G.
J Biol Chem. 1967 Apr 25;242(8):1802-14.
PubMed ID: 6024771
Identification and characterization of D-hydroxyproline dehydrogenase and Delta1-pyrroline-4-hydroxy-2-carboxylate deaminase involved in novel L-hydroxyproline metabolism of bacteria: metabolic convergent evolution.
Watanabe S(1), Morimoto D, Fukumori F, Shinomiya H, Nishiwaki H, Kawano-Kawada M, Sasai Y, Tozawa Y, Watanabe Y.
J Biol Chem. 2012 Sep 21;287(39):32674-88.
PubMed ID: 22833679