Pseudomonas aeruginosa PAO1, PA1509 (tplEi)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: tli4
Replicon chromosome
Genomic location 1638652 - 1639794 (- strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_250200.1
GI 15596706
Affymetrix PA1509_at
Entrez 879385
GenBank AAG04898.1
INSDC AAG04898.1
NCBI Locus Tag PA1509
protein_id(GenBank) gb|AAG04898.1|AE004579_10|gnl|PseudoCAP|PA1509
UniParc UPI00000C53B6
UniProtKB Acc Q9I3K3
UniRef100 UniRef100_Q9I3K3
UniRef50 UniRef50_Q9I3K3
UniRef90 UniRef90_Q9I3K3


Feature Type CDS
Coding Frame 1
Product Name
immunity protein, TplEi
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -0.91
Kyte-Doolittle Hydrophobicity Value -0.481
Molecular Weight (kDa) 42.4
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R1D HYDROLASE/HYDROLASE INHIBITOR 08/05/14 The crystal structure of Tle4-Tli4 complex PSEUDOMONAS AERUGINOSA PAO1 1.749 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003846 (291 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1509
Search term: tplEi

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 49156
Genomic Position 1638966
Position in ORF 315(1143)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 34800
Genomic Position 1639145
Position in ORF 494(1143)
Reference 14617778

Transposon Insertions in Orthologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr;496(7446):508-12
PubMed ID: 23552891
The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress.
Jiang F, Wang X, Wang B, Chen L, Zhao Z, Waterfield NR, Yang G, Jin Q
Cell Rep 2016 Aug;16(6):1502-9
PubMed ID: 27477276