Pseudomonas aeruginosa PAO1, PA1509 (tplEi)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: tli4
Replicon chromosome
Genomic location 1638652 - 1639794 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_250200.1
GI 15596706
Affymetrix PA1509_at
Entrez 879385
GenBank AAG04898.1
INSDC AAG04898.1
NCBI Locus Tag PA1509
protein_id(GenBank) gb|AAG04898.1|AE004579_10|gnl|PseudoCAP|PA1509
UniParc UPI00000C53B6
UniProtKB Acc Q9I3K3
UniRef100 UniRef100_Q9I3K3
UniRef50 UniRef50_Q9I3K3
UniRef90 UniRef90_Q9I3K3


Feature Type CDS
Coding Frame 1
Product Name
immunity protein, TplEi
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -0.91
Kyte-Doolittle Hydrophobicity Value -0.481
Molecular Weight (kDa) 42.4
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R1D HYDROLASE/HYDROLASE INHIBITOR 08/05/14 The crystal structure of Tle4-Tli4 complex PSEUDOMONAS AERUGINOSA PAO1 1.749 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003846 (292 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1509
Search term: tplEi

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 49156
Genomic Position 1638966
Position in ORF 315(1143)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 34800
Genomic Position 1639145
Position in ORF 494(1143)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_44920
Mutant ID PA14NR: 37856
Genomic Position 4005198
Position in ORF 179(1143)
Reference 16477005


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PubMed ID: 15687295
Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB(1), LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD.
Nature. 2013 Apr 25;496(7446):508-12.
PubMed ID: 23552891
The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress.
Jiang F(1), Wang X(1), Wang B(1), Chen L(1), Zhao Z(1), Waterfield NR(2), Yang G(3), Jin Q(4).
Cell Rep. 2016 Aug 9;16(6):1502-9.
PubMed ID: 27477276