Pseudomonas aeruginosa PAO1, PA2014 (liuB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2014
Name
liuB
Synonym: gnyB
Replicon chromosome
Genomic location 2203460 - 2205067 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250704.1
GI 15597210
Affymetrix PA2014_at
Entrez 878244
GenBank AAG05402.1
INSDC AAG05402.1
NCBI Locus Tag PA2014
protein_id(GenBank) gb|AAG05402.1|AE004627_10|gnl|PseudoCAP|PA2014
TIGR NTL03PA02014
UniParc UPI00000C555A
UniProtKB Acc Q9I297
UniProtKB ID Q9I297_PSEAE
UniRef100 UniRef100_W0YNW0
UniRef50 UniRef50_Q9LDD8
UniRef90 UniRef90_A0A024HIF6

Product

Feature Type CDS
Coding Frame 1
Product Name
methylcrotonyl-CoA carboxylase, beta-subunit
Product Name Confidence: Class 1
Synonyms MccB
beta subunit of geranoyl-CoA carboxylase, GnyB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.68
Kyte-Doolittle Hydrophobicity Value 0.021
Molecular Weight (kDa) 57.4
Isoelectric Point (pI) 6.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3U9T LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, free enzyme Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 100.0
3U9R LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0
3U9S LIGASE 10/19/11 Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, CoA complex Pseudomonas aeruginosa 3.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 398 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003425 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2014
Search term: liuB

Human Homologs

Ensembl 110, assembly GRCh38.p14
methylcrotonyl-CoA carboxylase subunit 2 [Source:HGNC Symbol;Acc:HGNC:6937]
E-value: 9.3e-206
Percent Identity: 65.3

References

The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa.
Díaz-Pérez AL, Zavala-Hernández AN, Cervantes C, Campos-García J
Appl Environ Microbiol 2004 Sep;70(9):5102-10
PubMed ID: 15345388
Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.
Förster-Fromme K, Höschle B, Mack C, Bott M, Armbruster W, Jendrossek D
Appl Environ Microbiol 2006 Jul;72(7):4819-28
PubMed ID: 16820476
An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae.
Philipp WJ, Poulet S, Eiglmeier K, Pascopella L, Balasubramanian V, Heym B, Bergh S, Bloom BR, Jacobs WR Jr, Cole ST
Proc Natl Acad Sci U S A 1996 Apr 2;93(7):3132-7
PubMed ID: 8610181