Pseudomonas aeruginosa PAO1, PA2080 (kynU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2080
Name
kynU
Replicon chromosome
Genomic location 2289085 - 2290335 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentGenetic evidence, using kynU knockout strain and analyzing PQS production. In ExPASy, this reaction is one of several in the EC #. Listed as kynureninase in NCBI.

Cross-References

RefSeq NP_250770.1
GI 15597276
Affymetrix PA2080_at
Entrez 877856
GenBank AAG05468.1
INSDC AAG05468.1
NCBI Locus Tag PA2080
protein_id(GenBank) gb|AAG05468.1|AE004635_6|gnl|PseudoCAP|PA2080
TIGR NTL03PA02080
UniParc UPI00000C558F
UniProtKB Acc Q9I235
UniProtKB ID Q9I235_PSEAE
UniRef100 UniRef100_Q9I235
UniRef50 UniRef50_P83788
UniRef90 UniRef90_Q9I235

Product

Feature Type CDS
Coding Frame 1
Product Name
kynureninase KynU
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.73
Kyte-Doolittle Hydrophobicity Value -0.125
Molecular Weight (kDa) 46.3
Isoelectric Point (pI) 5.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 361 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003375 (352 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2080
Search term: kynU
Search term: kynureninase KynU

Human Homologs

References

Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase.
Kurnasov O, Jablonski L, Polanuyer B, Dorrestein P, Begley T, Osterman A
FEMS Microbiol Lett 2003 Oct 24;227(2):219-27
PubMed ID: 14592712
Two distinct pathways supply anthranilate as a precursor of the Pseudomonas quinolone signal.
Farrow JM 3rd, Pesci EC
J Bacteriol 2007 May;189(9):3425-33
PubMed ID: 17337571