Pseudomonas aeruginosa PAO1, PA2524 (czcS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2524
Name
czcS
Replicon chromosome
Genomic location 2844489 - 2845907 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentThis two-component system is involved in cadmiun, zinc and cobalt resistance with homology to the CzcRS of Cupriavidus metallidurans.

Cross-References

RefSeq NP_251214.1
GI 15597720
Affymetrix PA2524_at
Entrez 878718
GenBank AAG05912.1
INSDC AAG05912.1
NCBI Locus Tag PA2524
protein_id(GenBank) gb|AAG05912.1|AE004679_6|gnl|PseudoCAP|PA2524
TIGR NTL03PA02524
UniParc UPI00000C571C
UniProtKB Acc Q9I0V9
UniProtKB ID Q9I0V9_PSEAE
UniRef100 UniRef100_Q9I0V9
UniRef50 UniRef50_Q02MX2
UniRef90 UniRef90_Q02MX2

Product

Feature Type CDS
Coding Frame 1
Product Name
CzcS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 0.57
Kyte-Doolittle Hydrophobicity Value -0.155
Molecular Weight (kDa) 51.9
Isoelectric Point (pI) 7.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5GPO TRANSFERASE 08/03/16 The sensor domain structure of the zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa Pseudomonas aeruginosa 1.701 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 618 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003042 (473 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2524
Search term: czcS
Search term: CzcS

Human Homologs

References

CzcR-CzcS, a two-component system involved in heavy metal and carbapenem resistance in Pseudomonas aeruginosa.
Perron K, Caille O, Rossier C, Van Delden C, Dumas JL, Köhler T
J Biol Chem 2004 Mar 5;279(10):8761-8
PubMed ID: 14679195
The transcriptional regulator CzcR modulates antibiotic resistance and quorum sensing in Pseudomonas aeruginosa.
Dieppois G, Ducret V, Caille O, Perron K
PLoS One 2012;7(5):e38148
PubMed ID: 22666466
A copper-activated two-component system interacts with zinc and imipenem resistance in Pseudomonas aeruginosa.
Caille O, Rossier C, Perron K
J Bacteriol 2007 Jul;189(13):4561-8
PubMed ID: 17449606