Pseudomonas aeruginosa PAO1, PA2934 (cif)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2934
Name
cif
Replicon chromosome
Genomic location 3289715 - 3290674 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 7 transposon mutants in orthologs

Cross-References

RefSeq NP_251624.1
GI 15598130
Affymetrix PA2934_at
DNASU PaCD00007278
Entrez 882654
GenBank AAG06322.1
INSDC AAG06322.1
NCBI Locus Tag PA2934
protein_id(GenBank) gb|AAG06322.1|AE004719_8|gnl|PseudoCAP|PA2934
TIGR NTL03PA02934
UniParc UPI00000C5891
UniProtKB Acc Q9HZR3
UniProtKB ID Q9HZR3_PSEAE
UniRef100 UniRef100_Q9HZR3
UniRef50 UniRef50_Q9HZR3
UniRef90 UniRef90_Q9HZR3

Product

Feature Type CDS
Coding Frame 1
Product Name
CFTR inhibitory factor, Cif
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -3.21
Kyte-Doolittle Hydrophobicity Value -0.157
Molecular Weight (kDa) 35.8
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5HKB Hydrolase/Hydrolase inhibitor 01/13/16 Crystal structure of the CFTR inhibitory factor Cif bound to the inhibitor KB2115 Pseudomonas aeruginosa UCBPP-PA14 1.65 X-RAY DIFFRACTION 99.3
5TNM HYDROLASE 10/14/16 Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted (R)-1,2-Epoxyoctane hydrolysis intermediate Pseudomonas aeruginosa (strain UCBPP-PA14) 1.7 X-RAY DIFFRACTION 99.0
5HK9 HYDROLASE/HYDROLASE INHIBITOR 01/13/16 Crystal structure of the CFTR inhibitory factor Cif bound to a urea inhibitor Pseudomonas aeruginosa (strain UCBPP-PA14) 1.8 X-RAY DIFFRACTION 99.3
5HKA Hydrolase/Hydrolase inhibitor 01/13/16 Crystal structure of the CFTR inhibitory factor Cif bound to an amide inhibitor Pseudomonas aeruginosa (strain UCBPP-PA14) 2.05 X-RAY DIFFRACTION 99.3
3KD2 HYDROLASE 10/22/09 Crystal structure of the CFTR inhibitory factor Cif Pseudomonas aeruginosa UCBPP-PA14 1.8 X-RAY DIFFRACTION 99.3
4YX9 Hydrolase/Hydrolase inhibitor 03/22/15 Crystal structure of the CFTR inhibitory factor Cif bound to tiratricol Pseudomonas aeruginosa (strain UCBPP-PA14) 1.75 X-RAY DIFFRACTION 99.3
4EUS HYDROLASE 04/25/12 Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol Pseudomonas aeruginosa 1.65 X-RAY DIFFRACTION 99.3

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Homo sapiens
Notes
Infection model Human airway epithelial cells (Calu-3)
Reference 16236828

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 302 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000798 (2691 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2934
Search term: cif

Human Homologs

Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5
Ensembl 110, assembly GRCh38.p14
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value: 8.5e-05
Percent Identity: 32.5

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cif is negatively regulated by the TetR family repressor CifR.
MacEachran DP, Stanton BA, O'Toole GA
Infect Immun 2008 Jul;76(7):3197-206
PubMed ID: 18458065
The Pseudomonas aeruginosa secreted protein PA2934 decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator.
MacEachran DP, Ye S, Bomberger JM, Hogan DA, Swiatecka-Urban A, Stanton BA, O'Toole GA
Infect Immun 2007 Aug;75(8):3902-12
PubMed ID: 17502391
Pseudomonas aeruginosa inhibits endocytic recycling of CFTR in polarized human airway epithelial cells.
Swiatecka-Urban A, Moreau-Marquis S, Maceachran DP, Connolly JP, Stanton CR, Su JR, Barnaby R, O'toole GA, Stanton BA
Am J Physiol Cell Physiol 2006 Mar;290(3):C862-72
PubMed ID: 16236828
Chemotoxicity of doxorubicin and surface expression of P-glycoprotein (MDR1) is regulated by the Pseudomonas aeruginosa toxin Cif.
Ye S, MacEachran DP, Hamilton JW, O'Toole GA, Stanton BA
Am J Physiol Cell Physiol 2008 Sep;295(3):C807-18
PubMed ID: 18650266
Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa.
Bahl CD, MacEachran DP, O'Toole GA, Madden DR
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Jan 1;66(Pt 1):26-8
PubMed ID: 20057063