Pseudomonas aeruginosa PAO1, PA3523 (mexP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3523
Name
mexP
Replicon chromosome
Genomic location 3942650 - 3943807 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_252213.1
GI 15598719
Affymetrix PA3523_at
DNASU PaCD00007550
Entrez 879790
GenBank AAG06911.1
INSDC AAG06911.1
NCBI Locus Tag PA3523
protein_id(GenBank) gb|AAG06911.1|AE004772_9|gnl|PseudoCAP|PA3523
TIGR NTL03PA03523
UniParc UPI00000C5A52
UniProtKB Acc Q9HY86
UniProtKB ID Q9HY86_PSEAE
UniRef100 UniRef100_Q4LDT7
UniRef50 UniRef50_Q4LDT7
UniRef90 UniRef90_Q4LDT7

Product

Feature Type CDS
Coding Frame 1
Product Name
MexP
Product Name Confidence: Class 3
Evidence for Translation
Charge (pH 7) 3.94
Kyte-Doolittle Hydrophobicity Value -0.114
Molecular Weight (kDa) 41.3
Isoelectric Point (pI) 9.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
mexP MexP is the membrane fusion protein of the MexPQ-OpmE multidrug efflux complex ARO:3003698 protein homolog model Perfect 0.0 1.0 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 422 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002142 (324 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3523
Search term: mexP
Search term: MexP

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 54898
Genomic Position 3943084
Position in ORF 435(1158)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_97_E3
Genomic Position 3942931
Position in ORF 282(1158)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 42174
Genomic Position 3943508
Position in ORF 859(1158)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa.
Mima T, Sekiya H, Mizushima T, Kuroda T, Tsuchiya T
Microbiol. Immunol. 2005 ;49(11):999-1002
PubMed ID: 16301811