Pseudomonas aeruginosa PAO1, PA4674

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4674
Name
Synonym: vapI
Replicon chromosome
Genomic location 5242254 - 5242559 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253363.1
GI 15599869
Affymetrix PA4674_at
DNASU PaCD00008030
Entrez 881405
GenBank AAG08061.1
INSDC AAG08061.1
NCBI Locus Tag PA4674
protein_id(GenBank) gb|AAG08061.1|AE004881_4|gnl|PseudoCAP|PA4674
TIGR NTL03PA04675
UniParc UPI00000C5DBA
UniProtKB Acc Q9HVC1
UniProtKB ID Q9HVC1_PSEAE
UniRef100 UniRef100_Q9HVC1
UniRef50 UniRef50_A6VCC3
UniRef90 UniRef90_A6VCC3

Product

Feature Type CDS
Coding Frame 1
Product Name
Antitoxin HigA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -3.29
Kyte-Doolittle Hydrophobicity Value -0.163
Molecular Weight (kDa) 11.2
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 217 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004324 (358 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4674
Search term: Antitoxin HigA

Transposon Insertions in PAO1

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_61840
Mutant ID PA14NR: 26343
Genomic Position 5514254
Position in ORF 59(306)
Reference 16477005

References

Genetic organization of the duplicated vap region of the Dichelobacter nodosus genome.
Katz ME, Wright CL, Gartside TS, Cheetham BF, Doidge CV, Moses EK, Rood JI.
J Bacteriol. 1994 May;176(9):2663-9.
PubMed ID: 8169216
The HigB/HigA toxin/antitoxin system of Pseudomonas aeruginosa influences the virulence factors pyochelin, pyocyanin, and biofilm formation.
Wood TL(1,)(2), Wood TK(1,)(3).
Microbiologyopen. 2016 Jun;5(3):499-511.
PubMed ID: 26987441