Pseudomonas aeruginosa PAO1, PA4709 (phuS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4709
Name
phuS
Synonym: phuS
Replicon chromosome
Genomic location 5287972 - 5289036 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Commentbelongs to Pseudomonas heme utilization (phu) operon

Cross-References

RefSeq NP_253397.1
GI 15599903
Affymetrix PA4709_at
Entrez 881544
GenBank AAG08095.1
INSDC AAG08095.1
NCBI Locus Tag PA4709
protein_id(GenBank) gb|AAG08095.1|AE004884_13|gnl|PseudoCAP|PA4709
TIGR NTL03PA04710
UniParc UPI00000C5DD7
UniProtKB Acc Q9HV89
UniProtKB ID Q9HV89_PSEAE
UniRef100 UniRef100_Q9HV89
UniRef50 UniRef50_O68880
UniRef90 UniRef90_O68880

Product

Feature Type CDS
Coding Frame 1
Product Name
PhuS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -9.18
Kyte-Doolittle Hydrophobicity Value -0.169
Molecular Weight (kDa) 39.1
Isoelectric Point (pI) 5.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4MGF METAL TRANSPORT 08/28/13 Crystal structure of apo-PhuS, a heme-binding protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 99.7
4IMH METAL TRANSPORT, TRANSPORT PROTEIN 01/02/13 Crystal Structure of Cytoplasmic Heme Binding Protein, PhuS, from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.976 X-RAY DIFFRACTION 99.7
4MF9 METAL TRANSPORT 08/27/13 Crystal structure of holo-PhuS, a heme-binding protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004356 (314 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4709
Search term: phuS
Search term: PhuS

Human Homologs

References

Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria.
Stojiljkovic I, Hantke K
EMBO J 1992 Dec;11(12):4359-67
PubMed ID: 1425573
Transport of haemin across the cytoplasmic membrane through a haemin-specific periplasmic binding-protein-dependent transport system in Yersinia enterocolitica.
Stojiljkovic I, Hantke K
Mol Microbiol 1994 Aug;13(4):719-32
PubMed ID: 7997183
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080
The cytoplasmic heme-binding protein (PhuS) from the heme uptake system of Pseudomonas aeruginosa is an intracellular heme-trafficking protein to the delta-regioselective heme oxygenase.
Lansky IB, Lukat-Rodgers GS, Block D, Rodgers KR, Ratliff M, Wilks A
J Biol Chem 2006 May 12;281(19):13652-13662
PubMed ID: 16533806
Genetics and regulation of two distinct haem-uptake systems, phu and has, in Pseudomonas aeruginosa.
Ochsner UA, Johnson Z, Vasil ML
Microbiology (Reading) 2000 Jan;146 ( Pt 1):185-198
PubMed ID: 10658665
Induced fit on heme binding to the Pseudomonas aeruginosa cytoplasmic protein (PhuS) drives interaction with heme oxygenase (HemO).
O'Neill MJ, Bhakta MN, Fleming KG, Wilks A
Proc Natl Acad Sci U S A 2012 Apr 10;109(15):5639-44
PubMed ID: 22451925
The P. aeruginosa heme binding protein PhuS is a heme oxygenase titratable regulator of heme uptake.
O'Neill MJ, Wilks A
ACS Chem Biol 2013 Aug 16;8(8):1794-802
PubMed ID: 23947366