Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4709
|
Name |
phuS
Synonym: phuS |
Replicon | chromosome |
Genomic location | 5287972 - 5289036 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Comment | belongs to Pseudomonas heme utilization (phu) operon |
RefSeq | NP_253397.1 |
GI | 15599903 |
Affymetrix | PA4709_at |
Entrez | 881544 |
GenBank | AAG08095.1 |
INSDC | AAG08095.1 |
NCBI Locus Tag | PA4709 |
protein_id(GenBank) | gb|AAG08095.1|AE004884_13|gnl|PseudoCAP|PA4709 |
TIGR | NTL03PA04710 |
UniParc | UPI00000C5DD7 |
UniProtKB Acc | Q9HV89 |
UniProtKB ID | Q9HV89_PSEAE |
UniRef100 | UniRef100_Q9HV89 |
UniRef50 | UniRef50_O68880 |
UniRef90 | UniRef90_O68880 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
PhuS
|
Synonyms | |
Evidence for Translation | |
Charge (pH 7) | -9.18 |
Kyte-Doolittle Hydrophobicity Value | -0.169 |
Molecular Weight (kDa) | 39.1 |
Isoelectric Point (pI) | 5.59 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4MGF | METAL TRANSPORT | 08/28/13 | Crystal structure of apo-PhuS, a heme-binding protein from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 99.7 |
4IMH | METAL TRANSPORT, TRANSPORT PROTEIN | 01/02/13 | Crystal Structure of Cytoplasmic Heme Binding Protein, PhuS, from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.976 | X-RAY DIFFRACTION | 99.7 |
4MF9 | METAL TRANSPORT | 08/27/13 | Crystal structure of holo-PhuS, a heme-binding protein from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.95 | X-RAY DIFFRACTION | 99.7 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004356 (314 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4709
Search term: phuS
Search term: PhuS
|
Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria.
Stojiljkovic I, Hantke K
EMBO J 1992 Dec;11(12):4359-67
PubMed ID: 1425573
|
Transport of haemin across the cytoplasmic membrane through a haemin-specific periplasmic binding-protein-dependent transport system in Yersinia enterocolitica.
Stojiljkovic I, Hantke K
Mol Microbiol 1994 Aug;13(4):719-32
PubMed ID: 7997183
|
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080
|
The cytoplasmic heme-binding protein (PhuS) from the heme uptake system of Pseudomonas aeruginosa is an intracellular heme-trafficking protein to the delta-regioselective heme oxygenase.
Lansky IB, Lukat-Rodgers GS, Block D, Rodgers KR, Ratliff M, Wilks A
J Biol Chem 2006 May 12;281(19):13652-13662
PubMed ID: 16533806
|
Genetics and regulation of two distinct haem-uptake systems, phu and has, in Pseudomonas aeruginosa.
Ochsner UA, Johnson Z, Vasil ML
Microbiology (Reading) 2000 Jan;146 ( Pt 1):185-198
PubMed ID: 10658665
|
Induced fit on heme binding to the Pseudomonas aeruginosa cytoplasmic protein (PhuS) drives interaction with heme oxygenase (HemO).
O'Neill MJ, Bhakta MN, Fleming KG, Wilks A
Proc Natl Acad Sci U S A 2012 Apr 10;109(15):5639-44
PubMed ID: 22451925
|
The P. aeruginosa heme binding protein PhuS is a heme oxygenase titratable regulator of heme uptake.
O'Neill MJ, Wilks A
ACS Chem Biol 2013 Aug 16;8(8):1794-802
PubMed ID: 23947366
|