Pseudomonas aeruginosa PAO1, PA5162 (rmlD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5162
Name
rmlD
Synonym: rfbD
Replicon chromosome
Genomic location 5811336 - 5812244 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253849.1
GI 15600355
Affymetrix PA5162_rmlD_at
Entrez 879983
GenBank AAG08547.1
INSDC AAG08547.1
NCBI Locus Tag PA5162
protein_id(GenBank) gb|AAG08547.1|AE004929_2|gnl|PseudoCAP|PA5162
TIGR NTL03PA05163
UniParc UPI00000DC6E2
UniProtKB Acc Q9HU23
UniProtKB ID Q9HU23_PSEAE
UniRef100 UniRef100_Q9HU23
UniRef50 UniRef50_Q48MY2
UniRef90 UniRef90_A6VDT3

Product

Feature Type CDS
Coding Frame 1
Product Name
dTDP-4-dehydrorhamnose reductase
Product Name Confidence: Class 2
Synonyms dTDP-L-rhamnose synthetase
dTDP-6-deoxy-L-mannose dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.92
Kyte-Doolittle Hydrophobicity Value -0.150
Molecular Weight (kDa) 33.1
Isoelectric Point (pI) 6.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 492 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004753 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5162
Search term: rmlD

Human Homologs

References

Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster.
Stevenson G, Neal B, Liu D, Hobbs M, Packer NH, Batley M, Redmond JW, Lindquist L, Reeves P
J Bacteriol 1994 Jul;176(13):4144-56
PubMed ID: 7517391
Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene.
Marolda CL, Valvano MA
J Bacteriol 1995 Oct;177(19):5539-46
PubMed ID: 7559340
Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar Typhimurium LT2.
Graninger M, Nidetzky B, Heinrichs DE, Whitfield C, Messner P
J Biol Chem 1999 Aug 27;274(35):25069-77
PubMed ID: 10455186
Purification, characterization and HPLC assay of Salmonella glucose-1-phosphate thymidylyl-transferase from the cloned rfbA gene.
Lindquist L, Kaiser R, Reeves PR, Lindberg AA
Eur J Biochem 1993 Feb 1;211(3):763-70
PubMed ID: 8382158